A variation of the amplified-fragment length polymorphism (AFLP) technique using three restriction endonucleases, and assessment of the enzyme combinationBglIIâMfeI for AFLP analysis ofSalmonella entericasubsp.entericaisolates
| Autor: | Traute Vardund, Even Heir, Georg Kapperud, Bjørn-Arne Lindstedt |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Salmonella typhimurium Salmonella Genotype Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Bacterial Proteins Genetics medicine Deoxyribonucleases Type II Site-Specific Molecular Biology Genotyping BglII Salmonella enterica DNA Restriction Enzymes bacterial infections and mycoses biology.organism_classification DNA Fingerprinting Bacterial Typing Techniques Restriction enzyme Salmonella enteritidis Evaluation Studies as Topic bacteria Salmonella enterica subsp. enterica Amplified fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | FEMS Microbiology Letters. 189:19-24 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2000.tb09200.x |
| Popis: | We have performed amplified-fragment length polymorphism (AFLP) fingerprinting on a collection of Salmonella enterica subsp. enterica serovar typhimurium strains with a restriction endonuclease combination (BglII and MfeI) that has previously been used successfully for typing Campylobacter jejuni isolates with high resolution. Additionally, a variation of the AFLP assay in which two rare cutting restriction enzymes (XbaI and BsrGI) in combination with the frequent cutter (HinP1I) was examined. The BglII and MfeI enzyme combination offered low resolution for genotyping Salmonella typhimurium isolates and is not recommended for this common serovar. The three-enzyme combination gave a higher discrimination, and is thus a new alternate way of performing AFLP fingerprinting of S. typhimurium. |
| Databáze: | OpenAIRE |
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