Aberrant methylation of the IL‐12B promotor region contributes to the risk of developing ovarian endometriosis
| Autor: | Yan Li, Lixian Wang, Jian Zhao, Wei Zhao, Shiru Cao, Shan Kang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Endometriosis Biology Endometrium Andrology 03 medical and health sciences 0302 clinical medicine Genetics medicine Humans Promoter Regions Genetic Gene Messenger RNA 030219 obstetrics & reproductive medicine Interleukin-12 Subunit p40 Aberrant methylation Promoter Cell Biology Methylation DNA Methylation Middle Aged medicine.disease female genital diseases and pregnancy complications 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Case-Control Studies Ovarian Endometriosis Female Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 86:632-638 |
| ISSN: | 1098-2795 1040-452X |
| DOI: | 10.1002/mrd.23139 |
| Popis: | Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian endometriosis. By using pyrosequencing, we analyzed the methylation level of the IL-12B promoter region in eutopic and ectopic endometrium of patients with ovarian endometriosis and normal endometrium of control women. The expression of IL-12B mRNA was detected by quantitative real-time PCR. The results showed that the methylation level of the IL-12B promoter region in ectopic and eutopic endometrium of patients with ovarian endometriosis was significantly lower than that in endometrium of women without endometriosis ( p < 0.001 and p = 0.041, respectively). In contrast, mRNA levels were significantly increased in ectopic and eutopic endometrium of patients with ovarian endometriosis compared to those in endometrium of women without endometriosis ( p < 0.001 and p = 0.042, respectively). Correlation analysis showed that the methylation level of the IL-12B promoter region was negatively correlated with mRNA levels of IL-12B ( p < 0.001). Our data suggested that aberrant methylation of the IL-12B promoter region may be responsible for aberrant IL-12B mRNA expression in endometrium tissue of women, which may be associated with the development of ovarian endometriosis in northern Chinese women. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text