MafA differentiates rat intestinal cells into insulin-producing cells
| Autor: | Hiroshi Maegawa, Tetsuya Hashimoto, Motoi Kudo, Atsunori Kashiwagi, Takaaki Nakamura, Satoshi Nomura, Yoshihiko Nishio |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Biophysics Biology Biochemistry law.invention Rats Sprague-Dawley Transcription (biology) In vivo law Insulin-Secreting Cells Internal medicine Diabetes mellitus Intestine Small medicine Animals Humans Insulin Lectins C-Type Intestinal Mucosa Molecular Biology Gene Membrane Glycoproteins Models Genetic Transcription Factor MafA Cell Differentiation Epithelial Cells Cell Biology medicine.disease Rats Endocrinology Hyperglycemia Recombinant DNA Immunohistochemistry |
| Zdroj: | Biochemical and Biophysical Research Communications. 349:136-143 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.08.032 |
| Popis: | Recent studies have suggested that basic leucine zipper transcription factor MafA has a crucial role in pancreatic β-cell-specific insulin gene transcription. Thus, we investigated whether MafA overexpression in the intestine induces insulin production in small-intestinal epithelial cells in vivo. Recombinant adenovirus containing MafA gene (Ad-MafA) was prepared and administered orally to streptozocin-treated diabetic rats. Insulin gene expression was observed in the intestine by RT-PCR analysis, and then insulin protein was detected by immunohistochemical analysis after Ad-MafA administration. Furthermore, MafA overexpression in the intestine increased plasma insulin levels and ameliorated hyperglycemia. These results indicate that MafA overexpression in the intestine induces intestinal epithelial cells newly to produce and release insulin. |
| Databáze: | OpenAIRE |
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