A suggested diagnostic approach for sporadic anthrax in cattle to protect public health
| Autor: | Darja Kušar, Jana Avberšek, Jasna Mićunović, Vasilij Cociancich, Mateja Pate, Silvio Špičić, Urška Zajc, Matjaž Ocepek, Sanja Duvnjak, Tomislav Paller |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
QH301-705.5 Spleen Multiple Loci VNTR Analysis Biology Microbiology 03 medical and health sciences Tandem repeat udc:636.09:616.9 Virology Genotype medicine diagnostics Biology (General) Genotyping multiple-locus variable-number tandem repeat analysis (MLVA) 030304 developmental biology 0303 health sciences 030306 microbiology Communication public health Outbreak anthrax biology.organism_classification Bacillus anthracis Real-time polymerase chain reaction medicine.anatomical_structure cattle real-time PCR |
| Zdroj: | Microorganisms, vol. 9, no. 8, 1567, 2021. Microorganisms, Vol 9, Iss 1567, p 1567 (2021) Microorganisms |
| ISSN: | 2076-2607 |
| Popis: | The repeated occurrence of anthrax in grazing animals should be a reminder of a widespread presence of Bacillus anthracis spores in the environment. Its rapid diagnosis is critical to protect public health. Here, we report a case of anthrax in cattle that was investigated using conventional and molecular methods. In 2015, six cows suddenly died within three days and the number of dead animals increased to a total of 12 within two weeks. At necropsy, anthrax was suspected. Therefore, spleen tissue samples were collected (from 6/12 animals) and laboratory tests (microscopy, cultivation, and real-time PCR) performed. The results of tissue staining for microscopy and cultivation were in congruence, while B. anthracis real-time PCR outperformed both. Spleen tissues from all six animals were real-time PCR-positive, while B. anthracis was successfully cultivated and detected by microscopy from the spleen of only three animals. Additionally, the ear tissue from another (1/12) cow tested positive by real-time PCR, supporting the suitability of ear clippings for molecular confirmation of B. anthracis. Genotyping of the isolates using multiple-locus variable-number tandem repeat analysis (MLVA) revealed a common source of infection as all three typed isolates had an indistinguishable MLVA genotype, which has not been observed previously in Europe. The results indicate that molecular testing should be selected as the first-line tool for confirming anthrax outbreaks in animals to ensure timely protection of public health. |
| Databáze: | OpenAIRE |
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