Caspase-3 immunohistochemical expression is a marker of apoptosis, increased grade and early recurrence in intracranial meningiomas
| Autor: | Hariklia Gakiopoulou, P Korkolopoulou, N. Givalos, George Agrogiannis, Xenophon Kotsiakis, E. Patsouris, H. Mahera, Anastasia E. Konstantinidou, Efstathios Boviatsis |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Receptors Steroid Cancer Research Pathology medicine.medical_specialty Mitotic index Clinical Biochemistry Pharmaceutical Science Apoptosis Caspase 3 Meningioma Meningeal Neoplasms medicine Animals Humans Survival rate Aged Pharmacology Enzyme Precursors biology Biochemistry (medical) Cell Biology Middle Aged medicine.disease Proliferating cell nuclear antigen Survival Rate Ki-67 biology.protein Immunohistochemistry Female Neoplasm Recurrence Local Biomarkers Immunostaining |
| Zdroj: | Apoptosis. 12:695-705 |
| ISSN: | 1573-675X 1360-8185 |
| DOI: | 10.1007/s10495-006-0001-4 |
| Popis: | Caspase-3 is the ultimate executioner caspase that is essential for the nuclear changes associated with apoptosis. We investigated caspase-3 immunohistochemical expression in 58 primary intracranial meningiomas, using one monoclonal antibody detecting both precursor and cleaved caspase-3 (CPP32) and a second recognizing only the cleaved activated form (ASP175). Caspase-3 expression was analyzed in relation to baseline apoptosis-as illustrated by the expression of anti-single stranded DNA (ss-DNA), the antiapoptotic protein bcl-2, proliferation indices (Ki-67, PCNA, topoisomerase IIa, mitosin C), hormonal status (estrogen, progesterone, androgen receptors), standard clinicopathological parameters and patients' disease-free survival. Caspase-3 immunostaining was observed in 62% of cases for CPP32 and in 24% for ASP175. In both instances, the labeling index (LI) was significantly correlated with ss-DNA LI (p=0.038 and p=0.018). CPP32 but not ASP175 LI positively correlated with the mitotic index (p=0.001) and PCNA LI (p=0.004). Both CPP32 and ASP175 LIs were increased in nonbenign meningiomas (p |
| Databáze: | OpenAIRE |
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