Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
| Autor: | Yoshinori Katakura, Kiichiro Teruya, Norihisa Uehara, Mizuki Ogawa, Miyako Udono |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
0301 basic medicine Flavonols exosomes Exosome Article Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Sirtuin 1 Hair cycle Skin Physiological Phenomena medicine Animals HaCaT Cells Humans Telomerase reverse transcriptase TX341-641 hair cycle regulation Telomerase Cell Proliferation Skin Nutrition and Dietetics integumentary system Cell growth Nutrition. Foods and food supply Stem Cells telogen–anagen transition telomerase reverse transcriptase keratinocyte–hair follicle stem cell interaction Hair follicle Microvesicles Cell biology Mice Inbred C57BL HaCaT 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Female Hair Follicle Fisetin Hair Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 2087, p 2087 (2021) Nutrients Volume 13 Issue 6 |
| ISSN: | 2072-6643 |
| Popis: | Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth. |
| Databáze: | OpenAIRE |
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