Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids
| Autor: | Solveig Sølverød Mo, Astrid Lousie Wester, Ståle Tofteland, Johanne Ahrenfeldt, Ole Lund, Jannice Schau Slettemeås, Marianne Sunde, Martin Steinbakk, Nils Grude, Ørjan Samuelsen, Einar Sverre Berg, Ulf R Dahle, Gunnar Skov Simonsen, Iren Høyland Löhr, Silje Bakken Jørgensen |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.drug_class 030106 microbiology Cephalosporin Zoonotic transfer of cephalosporin resistant E. coli Plasmid-mediated AmpC transmission Single-nucleotide polymorphism Biology medicine.disease_cause Genome Microbiology 03 medical and health sciences Plasmid Antibiotic resistance Chicken meat Medisinske Fag: 700 [VDP] medicine VDP::Medisinske Fag: 700 Artikkel SDG 2 - Zero Hunger Gene Escherichia coli Whole genome sequencing Zoonotic transfer of cephalosporin-resistant Escherichia coli General Medicine bacterial infections and mycoses Urinary tract infections Infectious Diseases |
| Zdroj: | Berg, E S, Wester, A L, Ahrenfeldt, J, Mo, S S, Slettemeås, J S, Steinbakk, M, Samuelsen, Grude, N, Simonsen, G S, Løhr, I H, Jørgensen, S B, Tofteland, S, Lund, O, Dahle, U R & Sunde, M 2017, ' Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/bla CMY-2 resistance plasmids ', Clinical Microbiology and Infection, vol. 23, no. 6, pp. 407.e9-407.e15 . https://doi.org/10.1016/j.cmi.2016.12.035 Berg, E S, Wester, A L, Ahrenfeldt, J, Mo, S S, Slettemeås, J S, Steinbakk, M, Samuelsen, Ø, Grude, N, Simonsen, G S, Løhr, I H, Jørgensen, S B, Tofteland, S, Lund, O, Dahle, U R & Sunde, M 2017, ' Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/bla CMY-2 resistance plasmids ', Clinical Microbiology and Infection, vol. 23, no. 6 . https://doi.org/10.1016/j.cmi.2016.12.035 |
| Popis: | Objectives In 2012 and 2014 the Norwegian monitoring programme for antimicrobial resistance in the veterinary and food production sectors (NORM-VET) showed that 124 of a total of 406 samples (31%) of Norwegian retail chicken meat were contaminated with extended-spectrum cephalosporin-resistant Escherichia coli. The aim of this study was to compare selected cephalosporin-resistant E. coli from humans and poultry to determine their genetic relatedness based on whole genome sequencing (WGS). Methods Escherichia coli representing three prevalent cephalosporin-resistant multi-locus sequence types (STs) isolated from poultry (n = 17) were selected from the NORM-VET strain collections. All strains carried an IncK plasmid with a blaCMY-2 gene. Clinical E. coli isolates (n = 284) with AmpC-mediated resistance were collected at Norwegian microbiology laboratories from 2010 to 2014. PCR screening showed that 29 of the clinical isolates harboured both IncK and blaCMY-2. All IncK/blaCMY-2-positive isolates were analysed with WGS-based bioinformatics tools. Results Analysis of single nucleotide polymorphisms (SNP) in 2.5 Mbp of shared genome sequences showed close relationship, with fewer than 15 SNP differences between five clinical isolates from urinary tract infections (UTIs) and the ST38 isolates from poultry. Furthermore, all of the 29 clinical isolates harboured IncK/blaCMY-2 plasmid variants highly similar to the IncK/blaCMY-2 plasmid present in the poultry isolates. Conclusions Our results provide support for the hypothesis that clonal transfer of cephalosporin-resistant E. coli from chicken meat to humans may occur, and may cause difficult-to-treat infections. Furthermore, these E. coli can be a source of AmpC-resistance plasmids for opportunistic pathogens in the human microbiota. |
| Databáze: | OpenAIRE |
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