Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease
| Autor: | Joon Sung Kim, Hyung Gil Kim, Yang-Kyu Choi, Byung-Wook Kim, Incheon, Tae-Geun Gweon, Chang Whan Kim, Jong-Hyung Park, Western Kyonggi Gastrointestinal Study, Jun-Won Chung, Sung Min Park |
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| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Esophageal Mucosa medicine.drug_class Proton-pump inhibitor Quinolones Gastroenterology 2-Pyridinylmethylsulfinylbenzimidazoles Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Western blot Internal medicine medicine Animals Rebamipide Pantoprazole Alanine Tight Junction Proteins Hepatology medicine.diagnostic_test Tight junction business.industry Anti-ulcer Agent Reflux Tight junction protein Drug Synergism Proton Pump Inhibitors Anti-Ulcer Agents medicine.disease Rats Disease Models Animal Gastroesophageal reflux 030220 oncology & carcinogenesis GERD Original Article Drug Therapy Combination 030211 gastroenterology & hepatology business medicine.drug |
| Zdroj: | Gut and Liver |
| ISSN: | 2005-1212 1976-2283 |
| DOI: | 10.5009/gnl17078 |
| Popis: | Background/Aims The aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD). Methods GERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups. An additional dose of rebamipide (100 mg/kg) was administered orally to the PPI+R group. Mucosal erosions, epithelial thickness, and leukocyte infiltration into the esophageal mucosa were measured in isolated esophagi 14 days after the procedure. A Western blot analysis was conducted to measure the expression of claudin-1, -3, and -4. Results The mean surface area of mucosal erosions, epithelial thickness, and leukocyte infiltration were lower in the PPI group and the PPI+R group than in the control group. Western blot analysis revealed that the expression of claudin-3 and -4 was significantly higher in the PPI+R group than in the control group. Conclusions Rebamipide may exert an additive effect in combination with PPI to modify the tight junction proteins of the esophageal mucosa in a rat model of GERD. This treatment might be associated with the relief of GERD symptoms. |
| Databáze: | OpenAIRE |
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