The miR166–SlHB15A regulatory module controls ovule development and parthenocarpic fruit set under adverse temperatures in tomato

Autor: Abdelhafid Bendahmane, Gwilherm Brisou, Marion Verdenaud, Ravi Sureshbhai Devani, Grégory Mouille, Halima Morin, Christian Clepet, Rachid Boumlik, Yanwei Hao, Adnane Boualem, Jean-Christophe Lepeltier, Sylvie Citerne, Brahim Mania, Fabien Marcel, Shai Koussevitzky
Přispěvatelé: Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), South China Agricultural University (SCAU), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), HM. CLAUSE [France], Hazera – Seeds of Growth, INRAE, Centre National de la Recherche Scientifique (CNRS) European Commission, Horizon 2020 research and innovation program (TOMRES)727929, ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), European Project: 0341076(2002)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Molecular Plant
Molecular Plant, Cell Press/Oxford UP, 2021, 14 (7), pp.1185-1198. ⟨10.1016/j.molp.2021.05.005⟩
Molecular Plant, 2021, 14 (7), pp.1185-1198. ⟨10.1016/j.molp.2021.05.005⟩
ISSN: 1674-2052
1752-9867
DOI: 10.1016/j.molp.2021.05.005⟩
Popis: International audience; Fruit set is inhibited by adverse temperatures, with consequences on yield. We isolated a tomato mutant producing fruits under non-permissive hot temperatures and identified the causal gene as SlHB15A, belonging to class III homeodomain leucine-zipper transcription factors. SlHB15A loss-of-function mutants display aberrant ovule development that mimics transcriptional changes occurring in fertilized ovules and leads to parthenocarpic fruit set under optimal and non-permissive temperatures, in field and greenhouse conditions. Under cold growing conditions, SlHB15A is subjected to conditional haploinsufficiency and recessive dosage sensitivity controlled by microRNA 166 (miR166). Knockdown of SlHB15A alleles by miR166 leads to a continuum of aberrant ovules correlating with parthenocarpic fruit set. Consistent with this, plants harboring an Slhb15a–miRNA166-resistant allele developed normal ovules and were unable to set parthenocarpic fruit under cold conditions. DNA affinity purification sequencing and RNA-sequencing analyses revealed that SlHB15A is a bifunctional transcription factor expressed in the ovule integument. SlHB15A binds to the promoters of auxin-related genes to repress auxin signaling and to the promoters of ethylene-related genes to activate their expression. A survey of tomato genetic biodiversity identified pat and pat-1, two historical parthenocarpic mutants, as alleles of SlHB15A. Taken together, our findings demonstrate the role of SlHB15A as a sentinel to prevent fruit set in the absence of fertilization and provide a mean to enhance fruiting under extreme temperatures.
Databáze: OpenAIRE
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