Reduced susceptibility of tomato stem to the necrotrophic fungus Botrytis cinerea is associated with a specific adjustment of fructose content in the host sugar pool

Autor: Manzoor Ali Abro, Astrid K. Raimbault, Félicie Lopez-Lauri, Philippe C. Nicot, Nadia Bertin, Julie Ripoll, François Lecompte
Přispěvatelé: Lecompte, François, Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Unité de Pathologie Végétale (PV), Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), ‘Agence Nationale de la Recherche’ project ANR-08-STRA-14, INRA programme ‘PICleg’, INRA division Environment and Agronomy, PhD fellowship of the Higher Education Commission of Pakistan, PhD fellow- ship of the Federative Research Structure Tersys, Station de Pathologie Végétale (AVI-PATHO), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Sucrose
Plant Science
champignon phytopathogène
01 natural sciences
fructose
chemistry.chemical_compound
Botrytis cinerea
Solanum lycopersicum
Plant Growth Regulators
sucre soluble
Food science
Jasmonate
glucose
Pathogen
pathologie végétale
2. Zero hunger
Abiotic component
Tomato (Solanum lycopersicum)
biology
Plant Stems
induction de résistance
food and beverages
necrotrophic fungi
plant defence
soluble sugars
hormones
mécanisme de défense de la plante
phytopathogenic fungus
Agricultural sciences
Botrytis
03 medical and health sciences
infection de la plante
Botany
soluble sugar
Sugar
Plant Diseases
fungi
Fructose
Original Articles
biology.organism_classification
030104 developmental biology
chemistry
Salicylic acid
Sciences agricoles
010606 plant biology & botany
Zdroj: Annals of Botany 5 (119), 931-943. (2017)
Annals of Botany
Annals of Botany, Oxford University Press (OUP), 2017, 119 (5), pp.931-943. ⟨10.1093/aob/mcw240⟩
ISSN: 0305-7364
1095-8290
DOI: 10.1093/aob/mcw240⟩
Popis: Background and aims Plant soluble sugars, as main components of primary metabolism, are thought to be implicated in defence against pathogenic fungi. However, the function of sucrose and hexoses remains unclear. This study aimed to identify robust patterns in the dynamics of soluble sugars in sink tissues of tomato plants during the course of infection by the necrotrophic fungus Botrytis cinerea. Distinct roles for glucose and fructose in defence against B. cinerea were hypothesized. [br/] Methods We examined sugar contents and defence hormonal markers in tomato stem tissues before and after infection by B. cinerea, in a range of abiotic environments created by various nitrogen and water supplies.[br/] Key Results Limited nitrogen or water supplies increased tomato stem susceptibility to B. cinerea. Glucose and fructose contents of tissues surrounding infection sites evolved differently after inoculation. The fructose content never decreased after inoculation with B. cinerea, while that of glucose showed either positive or negative variation, depending on the abiotic environment. An increase in the relative fructose content (defined as the proportion of fructose in the soluble sugar pool) was observed in the absence of glucose accumulation and was associated with lower susceptibility. A lower expression of the salicylic acid marker PR1a, and a lower repression of a jasmonate marker COI1 were associated with reduced susceptibility. Accordingly, COI1 expression was positively correlated with the relative fructose contents 7 d after infection. [br/] Conclusions Small variations of fructose content among the sugar pool are unlikely to affect intrinsic pathogen growth. Our results highlight distinct use of host glucose and fructose after infection by B. cinerea and suggest strongly that adjustment of the relative fructose content is required for enhanced plant defence.
Databáze: OpenAIRE
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