Involvement of lipid microdomains in human endothelial cells infected by Streptococcus agalactiae type III belonging to the hypervirulent ST-17
| Autor: | Marcelo Einicker-Lamas, Beatriz Jandre Ferreira, Gabriela da Silva Santos, Claudia Mermelstein, Pamella Silva Lannes-Costa, Prescilla Emy Nagao |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
caveolin-1 Membrane lipids RC955-962 030231 tropical medicine Biology medicine.disease_cause Microbiology Streptococcus agalactiae Membrane Lipids 03 medical and health sciences S. agalactiae HUVEC Membrane Microdomains 0302 clinical medicine Arctic medicine. Tropical medicine medicine Humans flotillin-1 Lipid raft PI3K/AKT/mTOR pathway lipid rafts Virulence Akt/PKB signaling pathway Lipid microdomain Infant Newborn Endothelial Cells QR1-502 Cell biology Endothelial stem cell PI3K/Akt pathway Caveolin 1 Original Article lipids (amino acids peptides and proteins) |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, 115:e190398 Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz. Memórias do Instituto Oswaldo Cruz, Volume: 115, Article number: e190398, Published: 16 MAR 2020 |
| Popis: | BACKGROUND Streptococcus agalactiae capsular type III strains are a leading cause of invasive neonatal infections. Many pathogens have developed mechanisms to escape from host defense response using the host membrane microdomain machinery. Lipid rafts play an important role in a variety of cellular functions and the benefit provided by interaction with lipid rafts can vary from one pathogen to another. OBJECTIVES This study aims to evaluate the involvement of membrane microdomains during infection of human endothelial cell by S. agalactiae. METHODS The effects of cholesterol depletion and PI3K/AKT signaling pathway activation during S. agalactiae-human umbilical vein endothelial cells (HUVEC) interaction were analysed by pre-treatment with methyl-β-cyclodextrin (MβCD) or LY294002 inhibitors, immunofluorescence and immunoblot analysis. The involvement of lipid rafts was analysed by colocalisation of bacteria with flotillin-1 and caveolin-1 using fluorescence confocal microscopy. FINDINGS In this work, we demonstrated the importance of the integrity of lipid rafts microdomains and activation of PI3K/Akt pathway during invasion of S. agalactiae strain to HUVEC cells. Our results suggest the involvement of flotillin-1 and caveolin-1 during the invasion of S. agalactiae strain in HUVEC cells. CONCLUSIONS The collection of our results suggests that lipid microdomain affects the interaction of S. agalactiae type III belonging to the hypervirulent ST-17 with HUVEC cells through PI3K/Akt signaling pathway. |
| Databáze: | OpenAIRE |
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