Carbonic anhydrase from Neisseria sicca, strain 6021 I. Bacterial growth and purification of the enzyme
| Autor: | L. Adler, J. Brundell, P.O. Nyman, S.O. Falkbring |
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| Rok vydání: | 1972 |
| Předmět: |
Cations
Divalent Buffers Neisseria sicca Bacterial growth Ammonium Chloride Drug Stability Carbonic anhydrase Polyacrylamide gel electrophoresis Carbonic Anhydrases chemistry.chemical_classification biology Strain (chemistry) Osmolar Concentration Sodium Temperature General Medicine Hydrogen-Ion Concentration Chromatography Ion Exchange Electrophoresis Disc biology.organism_classification Culture Media Kinetics Zinc Enzyme chemistry Biochemistry Chromatography Gel biology.protein Ultracentrifuge Neisseria Ultracentrifugation Bacteria |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Enzymology. 284:298-310 |
| ISSN: | 0005-2744 |
| DOI: | 10.1016/0005-2744(72)90068-x |
| Popis: | 1. 1.|Carbonic anhydrase (carbonate hydro-lyase, EC 4.2.1.1) from Neisseria sicca, strain 6021, has been studied. The bacteria have been grown in liquid cultures and the growth conditions have been varied in order to improve enzyme production. 2. 2.|The enzyme can be released from the bacteria by shaking the cells with glycine-NaOH buffer, pH 9–10. The extract has been fractionated and a procedure consisting of three ion-exchange chromatography steps is described giving more than 100-fold purification. 3. 3.|The purified enzyme appears to be homogeneous on ultracentrifugation and in polyacrylamide gel electrophoresis. No evidence has been obtained for the existence of more than one form of the bacterial enzyme; a situation in contrast to that for mammalian carbonic anhydrases where polymorphic patterns are often encountered. |
| Databáze: | OpenAIRE |
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