Biological functions of trout pavement-like gill cells in primary culture on solid support : pHi regulation, cell volume regulation and xenobiotic biotransformation
| Autor: | Jean Pierre Cravedi, M Pisam, Patrick Prunet, Isabelle Leguen |
|---|---|
| Přispěvatelé: | Station commune de Recherches en Ichtyophysiologie, Biodiversité et Environnement (SCRIBE), Institut National de la Recherche Agronomique (INRA), Xénobiotiques, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT), CEA- Saclay (CEA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Gills
Gill endocrine system animal structures Physiology 030310 physiology Intracellular pH Biology Biochemistry Calcium in biology Xenobiotics 03 medical and health sciences chemistry.chemical_compound Biotransformation SALMONIDAE ONCORHYNCHUS MYKISS Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology ECHANGE D'ION Cells Cultured ComputingMilieux_MISCELLANEOUS Cell Size 030304 developmental biology chemistry.chemical_classification 0303 health sciences Pavement cells Anatomy Hydrogen-Ion Concentration biology.organism_classification Cell biology Trout Enzyme chemistry CULTURE DE CELLULE Oncorhynchus mykiss Xenobiotic |
| Zdroj: | Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, 2001, 128 (2), pp.207-222. ⟨10.1016/S1095-6433(00)00293-2⟩ Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Elsevier, 2001, 128, pp.207-222 HAL |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/S1095-6433(00)00293-2⟩ |
| Popis: | International audience; This review presents results obtained on rainbow trout gill cells in primary culture on solid support. Ultrastructural analysis showed that cultured gill cells displayed features of pavement cells in situ. Several biological functions have been investigated on these cultured cells. First, it was shown that their intracellular pH at rest and after acidosis is regulated by a Na+/H+ exchanger. Second, gill cells in primary culture can regulate their volume after a cell swelling. Intracellular calcium appears to be involved in this regulation. The effects of different xenobiotics on the capacity of gill cells to regulate their volume are presented. Third, cultured pavement cells contain biotransformation enzymes to metabolize xenobiotics. All these results demonstrate that gill cells in primary culture on solid support represent a promising in vitro model for the study of pavement cells physiology, In conclusion, applications of this culture are discussed and compared with the permeable filter method, together with the limitations and prospects of this in vitro model on solid support. |
| Databáze: | OpenAIRE |
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