Docosahexaenoic acid slows inflammation resolution and impairs the quality of healed skin tissue
| Autor: | Erica M. Sernaglia, Helena L. Fisk, Felipe Cezar Pinheiro de Mato, Amanda Rabello Crisma, Marco Aurélio Ramirez Vinolo, Hosana G. Rodrigues, Beatriz Burger, Mariah B. P. Dos Anjos, Marcio Alberto Torsoni, Carolina M. C. Kuhl, Thamiris Candreva, Philip C. Calder, Sílvio Roberto Consonni |
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| Rok vydání: | 2019 |
| Předmět: |
Fatty Acid Desaturases
Male 0301 basic medicine Genetically modified mouse medicine.medical_specialty Docosahexaenoic Acids Neutrophils Mice Transgenic Endogeny Inflammation 03 medical and health sciences 0302 clinical medicine Internal medicine Edema medicine Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Skin Wound Healing biology Chemistry Macrophages General Medicine Fish oil 030104 developmental biology Endocrinology Docosahexaenoic acid Myeloperoxidase Dietary Supplements biology.protein Tumor necrosis factor alpha medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | Clinical Science. 133:2345-2360 |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs20190753 |
| Popis: | There is no consensus on the effects of omega-3 (ω-3) fatty acids (FA) on cutaneous repair. To solve this problem, we used 2 different approaches: (1) FAT-1 transgenic mice, capable of producing endogenous ω-3 FA; (2) wild-type (WT) mice orally supplemented with DHA-enriched fish oil. FAT-1 mice had higher systemic (serum) and local (skin tissue) ω-3 FA levels, mainly docosahexaenoic acid (DHA), in comparison with WT mice. FAT-1 mice had increased myeloperoxidase (MPO) activity and content of CXCL-1 and CXCL-2, and reduced IL-10 in the skin wound tissue three days after the wound induction. Inflammation was maintained by an elevated TNF-α concentration and presence of inflammatory cells and edema. Neutrophils and macrophages, isolated from FAT-1 mice, also produced increased TNF-α and reduced IL-10 levels. In these mice, the wound closure was delayed, with a wound area 6-fold bigger in relation with WT group, on the last day of analysis (14 days post-wounding). This was associated with poor orientation of collagen fibers and structural aspects in repaired tissue. Similarly, DHA group had a delay during late inflammatory phase. This group had increased TNF-α content and CD45+F4/80+ cells at the third day after skin wounding and increased concentrations of important metabolites derived from ω-3, like 18-HEPE, and reduced concentrations of those from ω-6 FA. In conclusion, elevated DHA content, achieved in both FAT-1 and DHA groups, slowed inflammation resolution and impaired the quality of healed skin tissue. |
| Databáze: | OpenAIRE |
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