Altered Steroidome in Women with Gestational Diabetes Mellitus: Focus on Neuroactive and Immunomodulatory Steroids from the 24th Week of Pregnancy to Labor

Autor: Marta Velíková, Michala Vosátková, Radmila Kancheva, Josef Bulant, Martin Hill, Kateřina Anderlová, Michaela Duskova, Michal Koucký, M. Vaňková, Leona Ondřejíková, Antonín Pařízek, Hana Krejčí, D. Vejražková, Patrik Šimják
Rok vydání: 2021
Předmět:
Male
medicine.medical_specialty
Neuroactive steroid
Chromatography
Gas
endocrine system diseases
immunoprotective steroids
mixed cord blood
neuroactive steroids
Biochemistry
Microbiology
Article
Pregnancy
Tandem Mass Spectrometry
Internal medicine
Medicine
Cytochrome P-450 CYP3A
Humans
Metabolomics
gestational age
Molecular Biology
20-Hydroxysteroid Dehydrogenases
Testosterone
gestational diabetes mellitus
steroidome
maternal blood
gas chromatography-tandem mass spectrometry
business.industry
Gestational age
nutritional and metabolic diseases
Steroid 17-alpha-Hydroxylase
medicine.disease
QR1-502
female genital diseases and pregnancy complications
Gestational diabetes
Diabetes
Gestational
Endocrinology
Pregnancy Trimester
Second
Gestation
HSD17B1
Female
Steroids
Cortisone
business
Oxidoreductases
medicine.drug
Zdroj: Biomolecules
Biomolecules; Volume 11; Issue 12; Pages: 1746
Biomolecules, Vol 11, Iss 1746, p 1746 (2021)
ISSN: 2218-273X
Popis: Gestational diabetes mellitus (GDM) is a complication in pregnancy, but studies focused on the steroidome in patients with GDM are not available in the public domain. This article evaluates the steroidome in GDM+ and GDM− women and its changes from 24 weeks (± of gestation) to labor. The study included GDM+ (n = 44) and GDM− women (n = 33), in weeks 24–28, 30–36 of gestation and at labor and mixed umbilical blood after delivery. Steroidomic data (101 steroids quantified by GC-MS/MS) support the concept that the increasing diabetogenic effects with the approaching term are associated with mounting progesterone levels. The GDM+ group showed lower levels of testosterone (due to reduced AKR1C3 activity), estradiol (due to a shift from the HSD17B1 towards HSD17B2 activity), 7-oxygenated androgens (competing with cortisone for HSD11B1 and shifting the balance from diabetogenic cortisol towards the inactive cortisone), reduced activities of SRD5As, and CYP17A1 in the hydroxylase but higher CYP17A1 activity in the lyase step. With the approaching term, the authors found rising activities of CYP3A7, AKR1C1, CYP17A1 in its hydroxylase step, but a decline in its lyase step, rising conjugation of neuroinhibitory and pregnancy-stabilizing steroids and weakening AKR1D1 activity.
Databáze: OpenAIRE
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