Sequence and in vitro expression of the phosphoprotein gene of avian pneumovirus
| Autor: | Roger Ling, Andrew J. Easton, Carla M. Wood, Philip J. Davis, Yu Qingzhong, Craig R. Pringle, David Cavanagh |
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| Rok vydání: | 1995 |
| Předmět: |
Gene Expression Regulation
Viral Avian Cancer Research Genes Viral Molecular Sequence Data Biology Article In vitro translation Conserved sequence Birds Virology Chlorocebus aethiops Animals Humans Amino Acid Sequence RNA Messenger Vero Cells Gene Peptide sequence Cells Cultured Conserved Sequence Genetics Sheep Base Sequence Pneumovirus Bird Diseases Phosphoproteins Molecular biology Respiratory Syncytial Viruses Avian pneumovirus Heptad repeat Open reading frame Infectious Diseases Protein Biosynthesis Phosphoprotein RNA Viral Cattle |
| Zdroj: | Virus Research |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/0168-1702(95)00008-e |
| Popis: | The phosphoprotein (P) gene of two subgroup A strains of avian pneumovirus comprised 855 nucleotides containing only one substantial open reading frame encoding a protein of 278 amino acids, with a predicted M(r) of 30,323. In vitro translation of P mRNA in a wheat germ system resulted in the synthesis of two polypeptides of M(r) 35,000. Comparison of the deduced P protein sequence with that of the known mammalian pneumoviruses revealed overall amino acid identities ranging from 31 to 34.5%, suggesting a distant relationship. However, there was a much higher identity (63.2-68.4%) in a region of 57 residues, which included a heptad repeat sequence. |
| Databáze: | OpenAIRE |
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