Direct Interaction of CD40 on Tumor Cells with CD40L on T Cells Increases the Proliferation of Tumor Cells by Enhancing TGF-β Production and Th17 Differentiation
| Autor: | Yejin Kim, Jun Man Hong, Jae Seung Kang, Seyeon Bae, Jiyea Choi, Wang Jae Lee, Mirim Jang, Jiwon Choi, Young Il Hwang, Hyemin Kim, Joo Myoung Kong |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
STAT3 Transcription Factor
T-Lymphocytes CD40 Ligand lcsh:Medicine Breast Neoplasms Biology Lymphocyte Activation Interleukin 21 Transforming Growth Factor beta Cancer stem cell Cell Line Tumor Humans Cytotoxic T cell RNA Messenger RNA Neoplasm IL-2 receptor CD40 Antigens RNA Small Interfering lcsh:Science skin and connective tissue diseases Cell Proliferation Interleukin 3 Multidisciplinary CD40 ZAP70 Interleukin-17 lcsh:R Cell Differentiation hemic and immune systems Coculture Techniques Up-Regulation Cell biology Interleukin 12 biology.protein Th17 Cells Female lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 5, p e0125742 (2015) PLoS ONE PLOS ONE(10): 5 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0125742 |
| Popis: | It has recently been reported that the CD40-CD40 ligand (CD40L) interaction is important in Th17 development. In addition, transforming growth factor-beta (TGF-beta) promotes tumorigenesis as an immunosuppressive cytokine and is crucial in the development of Th17 cells. This study investigated the role of CD40 in breast cancer cells and its role in immunosuppressive function and tumor progression. CD40 was highly expressed in the breast cancer cell line MDA-MB231, and its stimulation with CD40 antibodies caused the up-regulation of TGF-beta. Direct CD40-CD40L interaction between MDA-MB231 cells and activated T cells also increased TGF-beta production and induced the production of IL-17, which accelerated the proliferation of MDA-MB231 cells through the activation of STAT3. Taken together, the direct CD40-CD40L interaction of breast tumor cells and activated T cells increases TGF-beta production and the differentiation of Th17 cells, which promotes the proliferation of breast cancer cells. |
| Databáze: | OpenAIRE |
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