Expression of TNF-α system members in bovine ovarian follicles and the effects of TNF-α or dexamethasone on preantral follicle survival, development and ultrastructure in vitro
| Autor: | M.A.M. Donato, J.R.S. Passos, A. W. B. Silva, V.G. Menezes, R.P. Ribeiro, R. van den Hurk, J.J.N. Costa, Christina Alves Peixoto, P. B. D. Gonçalves, L.R.F. Melo, R.S. Barberino, M.H.T. Matos, A. M. P. Dau, F.T.G. Bezerra, J. R. V. Silva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cell Survival medicine.medical_treatment Apoptosis Biology Dexamethasone Tissue Culture Techniques 03 medical and health sciences Follicle 0302 clinical medicine Endocrinology Ovarian Follicle Food Animals Internal medicine medicine Animals RNA Messenger 030219 obstetrics & reproductive medicine Tumor Necrosis Factor-alpha Follicular atresia General Medicine Antral follicle In vitro 030104 developmental biology Cytokine Gene Expression Regulation Cattle Female Animal Science and Zoology Tumor necrosis factor alpha Folliculogenesis medicine.drug |
| Zdroj: | Animal Reproduction Science. 182:56-68 |
| ISSN: | 0378-4320 |
| DOI: | 10.1016/j.anireprosci.2017.04.010 |
| Popis: | This study was conducted to detect the protein expression of TNF-α system members (TNF-α/TNFR1/TNFR2) in bovine ovarian follicles and to evaluate the effects of TNF-α or dexamethasone on the survival and growth of primordial follicles in vitro, as well as on gene expression in cultured ovarian tissue. It was hypothesized that TNF-α induces follicular atresia in ovarian tissues cultured in vitro, and that dexamethasone suppresses the production of endogenous TNF-α, which can improve follicle viability in vitro. Ovarian fragments were cultured for 6days in α-MEM+ supplemented with TNF-α (0, 1, 10, 100 or 200ng/ml) or dexamethasone (0, 1, 10, 100 or 200ng/ml). After culture, the expression of mRNAs for BCL-2, BAX, P53, TNF-α, and CASP3 and CASP6 were evaluated. Immunohistochemical results showed that the TNF-α system members, were detected in bovine preantral and antral follicles. After 6days, the TNF-α (10ng/ml) treatment reduced the percentage of normal preantral follicles and increased the number of TUNEL-positive cells in cultured tissue. Dexamethasone (10ng/ml) during 6days of culture did maintain the percentage of normal follicles and the ultrastructure of follicles, while the presence of TNF-α or dexamethasone did not influence primordial follicle activation. However, TNF-α or dexamethasone had no effect on the levels of mRNA for P53, BCL-2, BAX and CASP6, in cultured tissues, but the presence of dexamethasone reduced the levels of CASP3 compared to ovarian slices cultured in control medium (α-MEM+). In conclusion, proteins of the TNF-α system are expressed at different bovine follicle stages. The addition of TNF-α in culture reduces follicle survival and increases the number of apoptotic cells in ovarian tissue, while the presence of dexamethasone maintains follicle ultrastructure in cultured tissue. |
| Databáze: | OpenAIRE |
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