Detection of alpha- and beta-human papillomavirus (HPV) in cutaneous melanoma: a matched and controlled study using specific multiplex PCR combined with DNA microarray primer extension

Autor: Jean Luc Prétet, Bernadette Kantelip, Christiane Mougin, Tarik Gheit, Jean Baptiste Ruer, Line Pépin, François Aubin, Chrystelle Vidal, Massimo Tommasino
Přispěvatelé: Laboratoire d'anatomie pathologique [Besancon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz-Université de Franche-Comté ( UFC ), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Université Libre de Bruxelles [Bruxelles] ( ULB ), Service d'Anatomie pathologique [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université libre de Bruxelles (ULB)
Rok vydání: 2009
Předmět:
Male
Oncology
Skin Neoplasms
Microarray
Gammapapillomavirus
MESH : Aged
MESH : Gammapapillomavirus
Alphapapillomavirus
Polymerase Chain Reaction
Biochemistry
[ SDV.CAN ] Life Sciences [q-bio]/Cancer
MESH: Aged
80 and over

0302 clinical medicine
MESH : Alphapapillomavirus
MESH : Neoplasm Staging
MESH : Female
MESH : DNA
Viral

Melanoma
MESH : Polymerase Chain Reaction
Oligonucleotide Array Sequence Analysis
Skin
MESH: Human papillomavirus 16
Aged
80 and over

MESH: Aged
Human papillomavirus 16
0303 health sciences
education.field_of_study
MESH: Middle Aged
MESH : Prognosis
HPV infection
virus diseases
MESH: Neoplasm Staging
Middle Aged
MESH : Adult
Prognosis
female genital diseases and pregnancy complications
3. Good health
MESH : Oligonucleotide Array Sequence Analysis
MESH: Young Adult
030220 oncology & carcinogenesis
Female
Adult
medicine.medical_specialty
MESH: Melanoma
MESH : Male
Population
MESH : Melanoma
MESH : Young Adult
[SDV.CAN]Life Sciences [q-bio]/Cancer
Dermatology
Biology
MESH: Betapapillomavirus
MESH: Prognosis
Young Adult
03 medical and health sciences
MESH: Skin
Internal medicine
Multiplex polymerase chain reaction
MESH : Skin
medicine
Betapapillomavirus
Humans
MESH : Middle Aged
MESH : Aged
80 and over

education
MESH: Alphapapillomavirus
Molecular Biology
Aged
Neoplasm Staging
030304 developmental biology
MESH: Humans
MESH : Betapapillomavirus
MESH: Skin Neoplasms
MESH : Skin Neoplasms
MESH : Humans
MESH : Human papillomavirus 16
Cancer
MESH: Adult
MESH: Polymerase Chain Reaction
medicine.disease
DNA extraction
MESH: Male
MESH: DNA
Viral

DNA
Viral

MESH: Oligonucleotide Array Sequence Analysis
Cutaneous melanoma
Immunology
MESH: Female
MESH: Gammapapillomavirus
Zdroj: Experimental Dermatology
Experimental Dermatology, Wiley, 2009, 18 (10), pp.857-62. 〈10.1111/j.1600-0625.2009.00866.x〉
Experimental Dermatology, Wiley, 2009, 18 (10), pp.857-62. ⟨10.1111/j.1600-0625.2009.00866.x⟩
ISSN: 1600-0625
0906-6705
DOI: 10.1111/j.1600-0625.2009.00866.x
Popis: International audience; There are few contradictory studies investigating the involvement of HPV in melanoma. We designed a controlled study to evaluate the HPV DNA prevalence in melanoma. One hundred patients with cutaneous malignant melanoma diagnosed between 2002 and 2006 were included. Complementary wide excision (healthy skin) was performed in 85 patients and was used as internal control. After DNA extraction, 68 different HPV types were studied using a multiplex PCR combined with microarray primer extension. We did not observe any statistical significant difference in terms of HPV DNA prevalence in melanoma (38.8%) and in healthy skin from wide excision (42.4%). Twenty-one different HPV types were detected but only one type was present in the majority of our samples (80/85 melanoma vs 59/66 HS). The distribution of HPV genera and types was similar in melanoma and HS, and beta-HPV was predominant (30.6% and 31.8%). Among alpha-HPV (10.6%), high-risk mucosal HPV16 was predominant. Among beta-HPV, melanoma harboured significantly more type 22 than control normal skin from the same patients and significantly less type 21 than paired control normal skin. No correlation between clinical and pathological melanoma characteristics and HPV DNA prevalence was found. Our data do not support a role of HPV infection in melanocarcinogenesis, but confirm the previous data suggesting that HPV DNA is widely distributed among the population and that occult HPV infections are frequent. Furthermore, specific HPV types, such as a-HPV16 and beta-HPV species 2 may be involved in a sub-group of melanoma.
Databáze: OpenAIRE
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