Detection of alpha- and beta-human papillomavirus (HPV) in cutaneous melanoma: a matched and controlled study using specific multiplex PCR combined with DNA microarray primer extension
Autor: | Jean Luc Prétet, Bernadette Kantelip, Christiane Mougin, Tarik Gheit, Jean Baptiste Ruer, Line Pépin, François Aubin, Chrystelle Vidal, Massimo Tommasino |
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Přispěvatelé: | Laboratoire d'anatomie pathologique [Besancon], Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Jean Minjoz-Université de Franche-Comté ( UFC ), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC ( CEF2P / CARCINO ), Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Université Bourgogne Franche-Comté ( UBFC ) -Université de Franche-Comté ( UFC ), Université Libre de Bruxelles [Bruxelles] ( ULB ), Service d'Anatomie pathologique [CHRU Besançon], Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), Carcinogénèse épithéliale : facteurs prédictifs et pronostiques - UFC (EA 3181) (CEF2P / CARCINO), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Université libre de Bruxelles (ULB) |
Rok vydání: | 2009 |
Předmět: |
Male
Oncology Skin Neoplasms Microarray Gammapapillomavirus MESH : Aged MESH : Gammapapillomavirus Alphapapillomavirus Polymerase Chain Reaction Biochemistry [ SDV.CAN ] Life Sciences [q-bio]/Cancer MESH: Aged 80 and over 0302 clinical medicine MESH : Alphapapillomavirus MESH : Neoplasm Staging MESH : Female MESH : DNA Viral Melanoma MESH : Polymerase Chain Reaction Oligonucleotide Array Sequence Analysis Skin MESH: Human papillomavirus 16 Aged 80 and over MESH: Aged Human papillomavirus 16 0303 health sciences education.field_of_study MESH: Middle Aged MESH : Prognosis HPV infection virus diseases MESH: Neoplasm Staging Middle Aged MESH : Adult Prognosis female genital diseases and pregnancy complications 3. Good health MESH : Oligonucleotide Array Sequence Analysis MESH: Young Adult 030220 oncology & carcinogenesis Female Adult medicine.medical_specialty MESH: Melanoma MESH : Male Population MESH : Melanoma MESH : Young Adult [SDV.CAN]Life Sciences [q-bio]/Cancer Dermatology Biology MESH: Betapapillomavirus MESH: Prognosis Young Adult 03 medical and health sciences MESH: Skin Internal medicine Multiplex polymerase chain reaction MESH : Skin medicine Betapapillomavirus Humans MESH : Middle Aged MESH : Aged 80 and over education MESH: Alphapapillomavirus Molecular Biology Aged Neoplasm Staging 030304 developmental biology MESH: Humans MESH : Betapapillomavirus MESH: Skin Neoplasms MESH : Skin Neoplasms MESH : Humans MESH : Human papillomavirus 16 Cancer MESH: Adult MESH: Polymerase Chain Reaction medicine.disease DNA extraction MESH: Male MESH: DNA Viral DNA Viral MESH: Oligonucleotide Array Sequence Analysis Cutaneous melanoma Immunology MESH: Female MESH: Gammapapillomavirus |
Zdroj: | Experimental Dermatology Experimental Dermatology, Wiley, 2009, 18 (10), pp.857-62. 〈10.1111/j.1600-0625.2009.00866.x〉 Experimental Dermatology, Wiley, 2009, 18 (10), pp.857-62. ⟨10.1111/j.1600-0625.2009.00866.x⟩ |
ISSN: | 1600-0625 0906-6705 |
DOI: | 10.1111/j.1600-0625.2009.00866.x |
Popis: | International audience; There are few contradictory studies investigating the involvement of HPV in melanoma. We designed a controlled study to evaluate the HPV DNA prevalence in melanoma. One hundred patients with cutaneous malignant melanoma diagnosed between 2002 and 2006 were included. Complementary wide excision (healthy skin) was performed in 85 patients and was used as internal control. After DNA extraction, 68 different HPV types were studied using a multiplex PCR combined with microarray primer extension. We did not observe any statistical significant difference in terms of HPV DNA prevalence in melanoma (38.8%) and in healthy skin from wide excision (42.4%). Twenty-one different HPV types were detected but only one type was present in the majority of our samples (80/85 melanoma vs 59/66 HS). The distribution of HPV genera and types was similar in melanoma and HS, and beta-HPV was predominant (30.6% and 31.8%). Among alpha-HPV (10.6%), high-risk mucosal HPV16 was predominant. Among beta-HPV, melanoma harboured significantly more type 22 than control normal skin from the same patients and significantly less type 21 than paired control normal skin. No correlation between clinical and pathological melanoma characteristics and HPV DNA prevalence was found. Our data do not support a role of HPV infection in melanocarcinogenesis, but confirm the previous data suggesting that HPV DNA is widely distributed among the population and that occult HPV infections are frequent. Furthermore, specific HPV types, such as a-HPV16 and beta-HPV species 2 may be involved in a sub-group of melanoma. |
Databáze: | OpenAIRE |
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