Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat
| Autor: | J D Butzner, V Dalal, R Parmar, C J Bell |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Colon medicine.medical_treatment Enema Stimulation Inflammation Butyrate digestive system Gastroenterology Internal medicine Animals Medicine Rats Wistar Colitis Saline Peroxidase Ion Transport Mucous Membrane biology business.industry Short-chain fatty acid medicine.disease digestive system diseases Rats Butyrates Disease Models Animal Trinitrobenzenesulfonic Acid Myeloperoxidase biology.protein medicine.symptom business Research Article |
| Zdroj: | Gut. 38:568-573 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.38.4.568 |
| Popis: | BACKGROUND--The short chain fatty acid (SCFA) butyrate provides energy for colonocytes, stimulates colonic fluid and electrolyte absorption and is recognised as an effective treatment for multiple types of colitis. AIM--To examine the impact of butyrate enema therapy on the clinical course, severity of inflammation, and SCFA stimulated Na+ absorption in a chronic experimental colitis. METHODS--Distal colitis was induced in rats with a trinitrobenzenesulphonic acid (TNBS) enema. Five days after induction, rats were divided into groups to receive: no treatment, saline enemas, or 100 mM Na-butyrate enemas daily. On day 24, colonic damage score and tissue myeloperoxidase (MPO) activity were evaluated. Colon was mounted in Ussing chambers and Na+ transport and electrical activities were measured during a basal period and after stimulation with 25 mM butyrate. RESULTS--In the untreated and the saline enema treated TNBS groups, diarrhoea and extensive colonic damage were seen, associated with increased tissue MPO activities and absent butyrate stimulated Na+ absorption. In contrast, in the butyrate enema treated TNBS group, diarrhoea ceased, colonic damage score improved, and tissue MPO activity as well as butyrate stimulated Na+ absorption recovered to control values. CONCLUSION--Butyrate enema therapy stimulated colonic repair, as evidenced by clinical recovery, decreased inflammation, and restoration of SCFA stimulated electrolyte absorption. |
| Databáze: | OpenAIRE |
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