In vitro evaluation of gelatin and chitosan electrospun fibres as an artificial guide in peripheral nerve repair: a comparative study

Autor: Rossella Laurano, S. Gnavi, Stefano Geuna, Chiara Tonda-Turo, Marco Zanetti, Benedetta Elena Fornasari, Gianluca Ciardelli
Jazyk: angličtina
Rok vydání: 2018
Předmět:
electrospun fibres
Sus scrofa
Nanofibers
Medicine (miscellaneous)
02 engineering and technology
Gelatin
Chitosan
chemistry.chemical_compound
0302 clinical medicine
Spectroscopy
Fourier Transform Infrared
Pseudopodia
Fiber
Axon
aligned fibers
Chemistry
random fibres
Cell Differentiation
Adhesion
021001 nanoscience & nanotechnology
Electrospinning
Actin Cytoskeleton
medicine.anatomical_structure
Female
0210 nano-technology
food.ingredient
electrospun fibers
Biomedical Engineering
macromolecular substances
Actin cytoskeleton organization
gelatin
Biomaterials
03 medical and health sciences
food
aligned fibres
Cell Adhesion
Neurites
medicine
Animals
Rats
Wistar
Cell adhesion
biomimetic materials
Cell Proliferation
Focal Adhesions
Tissue Engineering
chitosan
peripheral nerve repair
random fibers
Nerve Regeneration
Biophysics
Schwann Cells
030217 neurology & neurosurgery
Popis: Random and aligned gelatin (GL) and chitosan (CS) nano-fibres have been prepared by electrospinning tuning the collector rotation speed. The effect of fibre alignment on cell adhesion and proliferation was assessed in vitro by using different Schwann cell (SC) and neuronal models. Moreover, actin cytoskeleton organization, lamellipodia and filipodia formation, and axon outgrowth were evaluated. GL and CS fibres induced similar adhesion and proliferation rates. GL and CS random fibres promoted higher adhesion and proliferation rates induction in comparison to the aligned ones, although GL and CS fibres alignment resulted in SC and axon-oriented growth. Filipodia formation was higher on aligned fibres, suggesting that these substrates can promote higher cell migration in comparison to random ones. 50B11 (neuronal cell line) differentiation was higher on GL fibres, whereas no differences were observed in dorsal root ganglia explants model. These data suggest that both GL and CS fibres can be promising substrates to be used in peripheral nerve reconstruction. Copyright © 2016 John WileySons, Ltd.
Databáze: OpenAIRE
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