Bone Regeneration with Autologous Plasma, Bone Marrow Stromal Cells, and Porous β-Tricalcium Phosphate in Nonhuman Primates
| Autor: | Ichiro Torigoe, Atsushi Okawa, Kenichi Shinomiya, Yumi Sugata, Akio Tsuchiya, Shizuko Ichinose, Toshitaka Yoshii, Hidetsugu Maehara, Shinichi Sotome |
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| Rok vydání: | 2009 |
| Předmět: |
Calcium Phosphates
Male medicine.medical_specialty Bone Regeneration Stromal cell medicine.medical_treatment Cell Biomedical Engineering Bone Marrow Cells Bioengineering Fibrinogen Biochemistry Bone and Bones Fibrin Prosthesis Implantation Biomaterials Plasma chemistry.chemical_compound Osteogenesis Internal medicine medicine Animals Humans Bone regeneration Saline biology Phosphate Culture Media Macaca fascicularis medicine.anatomical_structure Endocrinology chemistry Immunology biology.protein Bone marrow Stromal Cells Porosity medicine.drug |
| Zdroj: | Tissue Engineering Part A. 15:1489-1499 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2008.0317 |
| Popis: | To potentiate the bone formation capability of bone marrow stromal cell (BMSC)/beta-tricalcium phosphate (beta-TCP) constructs, we devised an autologous plasma-based construct. We tested its effectiveness and investigated the effects of its components on a monkey ectopic bone formation model. The autologous plasma (platelet-rich plasma, PRP, or platelet-poor plasma, PPP)/BMSC/beta-TCP construct (R group or P group) showed significantly more bone formation at 3 and 6 weeks after implantation than a conventional BMSC/beta-TCP construct using a culture medium (M group). There was no significant difference between the P and R groups. Moreover, the P group constructs with a 10-fold lower cell concentration yielded equivalent bone formation to the M group at 5 weeks after implantation. To elucidate the effect of fibrin and serum contained in the plasma, five constructs were prepared using the following cell vehicles: autologous serum + fibrinogen (0, 1, 4, or 16 mg/mL) or phosphate-buffered saline + fibrinogen (4 mg/mL). The serum + fibrinogen (4 mg/mL, physiological concentration of monkeys) construct showed the most abundant bone formation at 3 weeks after implantation, though at 5 weeks no statistical difference existed among the groups. Autologous plasma efficiently promoted osteogenesis of BMSCs/porous beta-TCP constructs, and both fibrin and serum proved to play significant roles in the mechanism. |
| Databáze: | OpenAIRE |
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