The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440
| Autor: | Sofía Vieto, Max Chavarría, Felipe Vasquez-Castro, Rafael Montenegro, Daniela Wicki-Emmenegger, Paula Calderón, Reinaldo Pereira, Paola Fuentes-Schweizer, Carolina Coronado-Ruiz |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Strain (chemistry) 030306 microbiology Mutant PitB Wild type Transporter Oxyanion biology.organism_classification Phosphate Pseudonomas putida Microbiology Pseudomonas putida 03 medical and health sciences chemistry.chemical_compound Tellurite Enzyme Phosphate transporter Tellurium nanostwctures chemistry Biochemistry Bacteria 030304 developmental biology |
| Zdroj: | Microbiology, pp.1-9 Kérwá Universidad de Costa Rica instacron:UCR |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/mic.0.001002 |
| Popis: | Tellurium oxyanions are chemical species of great toxicity and their presence in the environment has increased because of mining industries and photovoltaic and electronic waste. Recovery strategies for this metalloid that are based on micro-organisms are of interest, but further studies of the transport systems and enzymes responsible for implementing tellurium transformations are required because many mechanisms remain unknown. Here, we investigated the involvement in tellurite uptake of the putative phosphate transporter PitB (PP1373) in soil bacterium Pseudomonas putida KT2440. For this purpose, through a method based on the CRISPR/Cas9 system, we generated a strain deficient in the pitB gene and characterized its phenotype on exposing it to varied concentrations of tellurite. Growth curves and transmission electronic microscopy experiments for the wild-type and ΔpitB strains showed that both were able to internalize tellurite into the cytoplasm and reduce the oxyanion to black nano-sized and rod-shaped tellurium particles, although the ΔpitB strain showed an increased resistance to the tellurite toxic effects. At a concentration of 100 μM tellurite, where the biomass formation of the wild-type strain decreased by half, we observed a greater ability of ΔpitB to reduce this oxyanion with respect to the wild-type strain (~38 vs ~16 %), which is related to the greater biomass production of ΔpitB and not to a greater consumption of tellurite per cell. The phenotype of the mutant was restored on over-expressing pitB in trans. In summary, our results indicate that PitB is one of several transporters responsible for tellurite uptake in P. putida KT2440. |
| Databáze: | OpenAIRE |
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