DNA nanotechnology-based composite-type gold nanoparticle-immunostimulatory DNA hydrogel for tumor photothermal immunotherapy
| Autor: | Yuka Umeki, Makiya Nishikawa, Shozo Ohtsuki, Tatsuya Murakami, Yoshinobu Takakura, Mengmeng Tan, Tomoya Yata, Hirotaka Nakatsuji, Tomoki Shiomi, Yuki Takahashi, Hiroshi Imahori |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
medicine.medical_treatment Biophysics Metal Nanoparticles Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Proinflammatory cytokine Biomaterials Interferon-gamma Immune system medicine Animals Nanotechnology Hydrogels DNA Immunotherapy Photothermal therapy 021001 nanoscience & nanotechnology Molecular biology 0104 chemical sciences Hsp70 Mechanics of Materials Colloidal gold Cancer cell Self-healing hydrogels Ceramics and Composites Cancer research CpG Islands Gold 0210 nano-technology |
| Zdroj: | Biomaterials. 146:136-145 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2017.09.014 |
| Popis: | Success of tumor photothermal immunotherapy requires a system that induces heat stress in cancer cells and enhances strong anti-tumor immune responses. Here, we designed a composite-type immunostimulatory DNA hydrogel consisting of a hexapod-like structured DNA (hexapodna) with CpG sequences and gold nanoparticles. Mixing of the properly designed hexapodna and oligodeoxynucleotide-modified gold nanoparticles resulted in the formation of composite-type gold nanoparticle-DNA hydrogels. Laser irradiation of the hydrogel resulted in the release of hexapodna, which efficiently stimulated immune cells to release proinflammatory cytokines. Then, EG7-OVA tumor-bearing mice received an intratumoral injection of a gold nanoparticle-DNA hydrogel, followed by laser irradiation at 780 nm. This treatment increased the local temperature and the mRNA expression of heat shock protein 70 in the tumor tissue, increased tumor-associated antigen-specific IgG levels in the serum, and induced tumor-associated antigen-specific interferon-γ production from splenocytes. Moreover, the treatment significantly retarded the tumor growth and extended the survival of the tumor-bearing mice. |
| Databáze: | OpenAIRE |
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