Effects of Decalcifying Agents of Variable Duration on PD-L1 Immunohistochemistry
| Autor: | John Markantonis, Elena Lucas, Kyle Molberg, Sara Blacketer, Amanda L. Strickland |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Formates Formic acid Placenta B7-H1 Antigen Programmed cell death ligand 1 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy PD-L1 Humans Prospective Studies Lung Edetic Acid Calcium Chelating Agents Bone decalcification biology Chemistry General Medicine Immunohistochemistry Molecular biology Staining 030104 developmental biology 030220 oncology & carcinogenesis biology.protein Female Hydrochloric Acid |
| Zdroj: | American Journal of Clinical Pathology. 153:258-265 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/aqz161 |
| Popis: | Objectives To evaluate the effects of decalcifying agents on programmed cell death ligand 1 (PD-L1) immunohistochemistry (IHC). Methods Fragments of 10 placentas (high PD-L1 expressor) and 10 lungs (lower PD-L1 expressor) were formalin-fixed and subjected to four decalcifying solutions (EDTA, formic acid/MasterCal IM Plus [FA/MC], 12% HCl, and Decal STAT/23% HCl) for 1, 2, 6, or 24 hours. H&E staining and PD-L1 using IHC 22C3 pharmDx were performed, and PD-L1 staining was assessed. Results Minimal to no change in staining intensity or proportion of stained cells was seen with EDTA or FA/MC at all decalcifying durations. Both HCl-based decalcifiers demonstrated a progressive decrease in percentage of positive cells and staining intensity with longer decalcifying duration, particularly with Decal STAT. Conclusions EDTA and FA/MC have little effect on PD-L1 expression. 12% HCl causes a progressive decline in staining. Decal STAT dramatically reduced staining with all treatment durations, especially at 24 hours. |
| Databáze: | OpenAIRE |
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