Cloning and Chromosomal Mapping of Three Novel Genes, GPR9, GPR10, and GPR14, Encoding Receptors Related to Interleukin 8, Neuropeptide Y, and Somatostatin Receptors
| Autor: | Adriano Marchese, Xiao-Mei Shi, Victor Saldivia, Paul Gregor, Philip M. Murphy, Tuan V. Nguyen, John Docherty, Regina Cheng, Henry H.Q. Heng, Brian F. O'Dowd, Lap-Chee Tsui, Michael Heiber, Susan R. George |
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| Rok vydání: | 1995 |
| Předmět: |
Neuropeptide Y receptor Y1
Neuropeptide Y receptor Y2 Molecular Sequence Data Biology Polymerase Chain Reaction Receptors Interleukin-8A Antigens CD Genetics Somatostatin receptor 3 Humans Somatostatin receptor 1 5-HT5A receptor Amino Acid Sequence Lymphocytes RNA Messenger Receptors Somatostatin Cloning Molecular GABBR1 In Situ Hybridization Fluorescence Gene Library Base Sequence Sequence Homology Amino Acid Somatostatin receptor Interleukin-8 Brain Chromosome Mapping DNA Receptors Interleukin Blotting Northern Molecular biology Recombinant Proteins Receptors Neuropeptide Y Transmembrane domain Receptors Opioid Chromosomes Human Pair 8 |
| Zdroj: | Genomics. 29:335-344 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1995.9996 |
| Popis: | We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26. |
| Databáze: | OpenAIRE |
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