| Autor: |
Joseph Sushil Rao, John C. Bischof, Paul A. Iaizzo, Michael Garwood, Hattie L. Ring, Elena G. Tolkacheva, Erik B. Finger, Zhe Gao, Yoed Rabin, Baterdene Namsrai, Djaudat S. Idiyatullin, Purva Joshi, Elliott C. Magnuson, Michael L. Etheridge, Zonghu Han, Vasanth Ravikumar, Anirudh Sharma |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Advanced materials technologies. 7(3) |
| ISSN: |
2365-709X |
| Popis: |
To extend the preservation of donor hearts beyond the current 4-6 h, this paper explores heart cryopreservation by vitrification-cryogenic storage in a glass-like state. While organ vitrification is made possible by using cryoprotective agents (CPA) that inhibit ice during cooling, failure occurs during convective rewarming due to slow and non-uniform rewarming which causes ice crystallization and/or cracking. Here an alternative, "nanowarming", which uses silica-coated iron oxide nanoparticles (sIONPs) perfusion loaded through the vasculature is explored, that allows a radiofrequency coil to rewarm the organ quickly and uniformly to avoid convective failures. Nanowarming has been applied to cells and tissues, and a proof of principle study suggests it is possible in the heart, but proper physical and biological characterization especially in organs is still lacking. Here, using a rat heart model, controlled machine perfusion loading and unloading of CPA and sIONPs, cooling to a vitrified state, and fast and uniform nanowarming without crystallization or cracking is demonstrated. Further, nanowarmed hearts maintain histologic appearance and endothelial integrity superior to convective rewarming and indistinguishable from CPA load/unload control hearts while showing some promising organ-level (electrical) functional activity. This work demonstrates physically successful heart vitrification and nanowarming and that biological outcomes can be expected to improve by reducing or eliminating CPA toxicity during loading and unloading. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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