In Situ Akt Phosphorylation in the Nucleus Tractus Solitarii Is Involved in Central Control of Blood Pressure and Heart Rate

Autor: Ching-Jiunn Tseng, Pei Jung Lu, Michael Hsiao, Hsiao Ning Huang, Wan Chen Lo, Chia Hui Lin
Rok vydání: 2004
Předmět:
Male
medicine.medical_specialty
Nitric Oxide Synthase Type III
Morpholines
medicine.medical_treatment
Blood Pressure
Protein Serine-Threonine Kinases
Baroreflex
Nitric Oxide
Endothelial NOS
Rats
Inbred WKY
Nitric oxide
Cardiovascular Physiological Phenomena
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
Heart Rate
Proto-Oncogene Proteins
Physiology (medical)
Internal medicine
Bradycardia
Solitary Nucleus
medicine
Animals
Insulin
Phosphorylation
Microinjection
PI3K/AKT/mTOR pathway
Phosphoinositide-3 Kinase Inhibitors
omega-N-Methylarginine
biology
business.industry
Rats
Nitric oxide synthase
Insulin receptor
NG-Nitroarginine Methyl Ester
Endocrinology
nervous system
chemistry
Chromones
biology.protein
Nitric Oxide Synthase
Cardiology and Cardiovascular Medicine
business
Protein Processing
Post-Translational
Proto-Oncogene Proteins c-akt
Signal Transduction
Zdroj: Circulation. 110:2476-2483
ISSN: 1524-4539
0009-7322
DOI: 10.1161/01.cir.0000145116.75657.2d
Popis: Background— Previously, we have shown that nitric oxide (NO) plays a significant role in central cardiovascular regulation and modulates the baroreflex in the nucleus tractus solitarii (NTS) of rats. NO production is mediated by activation of NO synthase (NOS). Insulin signaling was involved in controlling peripheral blood pressure via the activation of endothelial NOS. Here, we investigated whether the insulin signal transduction pathway is involved in controlling central cardiovascular effects. Methods and Results— Insulin was injected into NTS of urethane-anesthetized male Wistar-Kyoto (WKY) rats. Unilateral microinjection (60 nL) of insulin (100 IU/mL) into the NTS produced prominent depressor and bradycardic effects in 8- and 16-week-old WKY rats. In addition, pretreatment with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 and the NOS inhibitor L-NAME into the NTS caused attenuation of the cardiovascular response evoked by insulin in either 8- or 16-week-old WKY rats. Western blot analysis showed a significant increase (2.6±0.4-fold; P Conclusions— Take together, these results suggest that the insulin-PI3K-Akt-NOS signaling pathway may play a significant role in central cardiovascular regulation.
Databáze: OpenAIRE
Externí odkaz: