| Popis: |
Endocytic organelles maintain their acidic pH using the V-type ATPase proton pump. However, proton accumulation across the membrane generates a voltage and requires the movement of an additional ion, known as a counterion, to dissipate charge buildup. The role of counterion movement in endosomes is not clear, but a sub-population of early endosomes, Clathrin-coated vesicles (CCVs), has previously been shown to use external chloride (Cl(−)) to allow V-ATPase-dependent acidification. We aimed to determine the identity and function of this presumed Cl(−) transporting protein. Our sample of highly enriched bovine brain CCVs exhibited V-type ATPase facilitated acidification in the presence of external Cl(−), independent of the monovalent cations present. While unsuccessful at identifying the mechanism of anion transport, we used glutamate-facilitated acidification, density gradients, and mass spectrometry to show that most brain CCVs are synaptic vesicles, complementing results from earlier studies that argued similarity only on the basis on protein content. The source of Cl(−)-dependent acidification in brain CCVs may be vGLUT1, a synaptic vesicle glutamate transporter with known Cl(−) permeability, although CCVs in other tissues are likely to utilize different proteins to facilitate acidification. |
