Sclerotinia Sclerotiorum (White Mold): Cytotoxic, Mutagenic, and Antimalarial Effects In Vivo and In Vitro
| Autor: | Diego M. Assis, Mario F.C. Santos, Mariana Araújo Vieira do Carmo, Daniela Aparecida Chagas de Paula, Marcos José Marques, Carolina Girotto Pressete, José da Cruz Machado, Marisi G. Soares, Laila Santos Vieira Giannini, Luciana Azevedo |
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| Rok vydání: | 2019 |
| Předmět: |
030309 nutrition & dietetics
Cell Survival Pharmacology medicine.disease_cause Mass Spectrometry 03 medical and health sciences Antimalarials 0404 agricultural biotechnology Ascomycota In vivo Cell Line Tumor medicine Humans Cytotoxicity Cells Cultured Chromatography High Pressure Liquid chemistry.chemical_classification 0303 health sciences Reactive oxygen species Biological Products biology Chemistry Sclerotinia sclerotiorum 04 agricultural and veterinary sciences biology.organism_classification 040401 food science In vitro Cancer cell Lipid Peroxidation Reactive Oxygen Species Oxidative stress Genotoxicity Food Science Mutagens |
| Zdroj: | Journal of food scienceReferences. 84(12) |
| ISSN: | 1750-3841 |
| Popis: | This work aimed includes performing the sclerotia chemical profile and evaluates their biological effects on mutagenesis, oxidative stress, cancer, and malaria. A chemical profile was determined by ultraperformance liquid chromatography mass spectrometry (UHPLC-HRMS) analysis dereplicating norditerpenoid dilactone, sclerolide, and other compounds. The GI50 values to cancer cells (19.8 to 277.6 µg/mL) were higher than normal (16.05 µg/mL), meaning high cytotoxicity. Regarding the oxidative stress, the results showed that the all AcOET fraction concentrations tested on IMR90 noncancer cell increased reactive oxygen species (ROS) production in more intense way (by fivefold) than in tested cancer cells. The in vivo study showed an increase of the following biomarkers (by 296.00%): % DNA in comet tail in peripheral blood and liver cells; micronucleated erythrocytes and colon cells and lipid serum peroxidation. These results indicate the sclerotia as genotoxic and mutagenic agent and its contamination may lead to fungal toxic effects with a risk to human health. |
| Databáze: | OpenAIRE |
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