Dendritic planarity of Purkinje cells is independent of Reelin signaling
| Autor: | Jinkyung Kim, Tom Curran, Tae-Ju Park, Jung Ho Je, Dongmyeong Lee, Kyong-Tai Kim, Tetsuya Ishikawa, Namseop Kwon, Seunghwan Kim, Yoshiki Kohmura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cerebellum
Calbindins Silver Staining Histology Neuroscience(all) Cell Adhesion Molecules Neuronal Purkinje cell Dendrite Nerve Tissue Proteins Biology Calbindin Adapter molecule crk Mice Purkinje Cells Imaging Three-Dimensional Cell Movement Dendritic planarity medicine Animals Reelin Migration Adaptor Proteins Signal Transducing Mice Knockout Brain Mapping Extracellular Matrix Proteins General Neuroscience Serine Endopeptidases Nuclear Proteins Dendrites Proto-Oncogene Proteins c-crk Synchrotron X-ray imaging Cell biology CRKL Reelin Protein medicine.anatomical_structure nervous system Reelin signaling Gene Expression Regulation biology.protein Original Article Signal transduction Anatomy Tomography X-Ray Computed Signal Transduction |
| Zdroj: | Brain Structure & Function |
| ISSN: | 1863-2661 1863-2653 |
| Popis: | The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation. Electronic supplementary material The online version of this article (doi:10.1007/s00429-014-0780-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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