CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells

Autor: Víctor Manuel Barberá, P. Garcia-Morales, Maria Fuentes-Baile, Daniel Bello-Gil, Miguel Saceda, María Paz Ventero, Elizabeth Pérez-Valenciano, Cristina Alenda, Jesús Sanz, Beatriz Maestro
Přispěvatelé: Instituto de Salud Carlos III, Asociación de Mujeres afectadas por Cáncer de mama de Elche y comarca, Ministerio de Economía, Industria y Competitividad (España), Consejo Superior de Investigaciones Científicas (España), Fuentes-Baile, María, Bello-Gil, Daniel, Sanz, Jesús M., Maestro, Beatriz, Ventero, María Paz, Alenda, Cristina, Barberá, Victor M., Saceda, Miguel, Fuentes-Baile, María [0000-0003-3653-2407], Bello-Gil, Daniel [0000-0002-0417-4513], Sanz, Jesús M. [0000-0002-4421-9376], Maestro, Beatriz [0000-0001-5317-650X], Ventero, María Paz [0000-0003-1979-0949], Alenda, Cristina [0000-0002-0560-1759], Barberá, Victor M. [0000-0002-4012-6973], Saceda, Miguel [0000-0002-1564-3602]
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Necrosis
DNA Repair
magnetic nanoparticle
lcsh:QR1-502
Apoptosis
oxidative damage
Biochemistry
lcsh:Microbiology
Choline
Mice
0302 clinical medicine
Cytotoxicity
Magnetite Nanoparticles
chemistry.chemical_classification
reactive oxygen species
Brain Neoplasms
N-Acetylmuramoyl-L-alanine Amidase
030220 oncology & carcinogenesis
Colonic Neoplasms
Adenocarcinoma
cancer therapy
cytotoxicity
medicine.symptom
Epigenetic therapy
choline-binding proteins
D-Amino-Acid Oxidase
Programmed cell death
DNA repair
Cell Survival
Article
03 medical and health sciences
Inhibitory Concentration 50
3T3-L1 Cells
Cell Line
Tumor
medicine
Animals
Humans
Molecular Biology
Reactive oxygen species
medicine.disease
Pancreatic Neoplasms
030104 developmental biology
chemistry
Cell culture
Cancer research
Glioblastoma
Magnetic nanoparticle
DNA Damage
Zdroj: Biomolecules, Vol 10, Iss 2, p 222 (2020)
Biomolecules
Volume 10
Issue 2
r-FISABIO. Repositorio Institucional de Producción Científica
instname
r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante
Digital.CSIC. Repositorio Institucional del CSIC
r-FISABIO: Repositorio Institucional de Producción Científica
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
ISSN: 2218-273X
Popis: 19 p.-8 fig.-1 tab.
D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.
This research was funded by Spanish Instituto de Salud Carlos III, grant number PI01202025, and donations from Association of women affected by breast cancer in Elche and the region (AMACMEC) to MS and grants from the Spanish Ministry of Economy, Industry and Competitiveness, grant numbers BIO2013-47684-R and BIO2016-79323-R, and the RETICS-FEDER RICET, RD16/0027/0010, to JMS. The CIBER of Enfermedades Respiratorias (CIBERES) is an initiative of the Spanish Instituto de Salud Carlos III and Spanish National Research Council (CSIC Grant 201820I132).
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje