Revised structure of a trehalose-containing immunoreactive glycolipid of Mycobacterium tuberculosis

Autor:	A, Lemassu, M A, Lanéelle, M, Daffé
Rok vydání:	1991
Předmět:	Antigens Bacterial Chromatography Gas Magnetic Resonance Spectroscopy Molecular Structure Genetics Trehalose Chromatography Thin Layer Mycobacterium tuberculosis Glycolipids Spectrometry Mass Fast Atom Bombardment Molecular Biology Microbiology
Zdroj:	FEMS Microbiology Letters. 78:171-175
ISSN:	0378-1097
DOI:	10.1016/0378-1097(91)90153-2
Popis:	Nuclear magnetic resonance spectroscopy, fast-atom bombardment mass spectrometry as well as various chemical degradations and chromatographic techniques were used to re-examine the structure of a highly immunoreactive glycolipid previously described in Mycobacterium tuberculosis (strain Canetti) as a 2,3-diacyl trehalose 2'-sulfate (labelled SL-IV). Ion exchange chromatography allowed the recognition of a neutral and an acidic glycolipid, indistinguishable on conventional silica gel. The neutral glycolipid was shown to be serologically identical to SL-IV and its structure was established as 2,3-diacyl trehalose. It corresponded to the non-chemically defined highly observed immunoreactive lipid previously recognized by others in M. tuberculosis (H37Rv).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4095f707ae8209c4a6c2918a390b4444 https://doi.org/10.1016/0378-1097(91)90153-2 Zobrazit plný text záznamu Plný text