Migratory capacity and function of dendritic cells from mesenteric afferent lymph nodes after feeding a single dose of vitamin A

Autor: Lisa Maccio-Maretto, Ivanna Novotny Nuñez, Silvia G. Correa, Bibiana E. Barrios
Jazyk: angličtina
Rok vydání: 2017
Předmět:
CD4-Positive T-Lymphocytes
0301 basic medicine
Nutrición
Dietética
Endocrinology
Diabetes and Metabolism
Receptor expression
Clinical Biochemistry
Ciencias de la Salud
Biochemistry
0302 clinical medicine
Cell Movement
Mesenteric lymph nodes
Mesentery
Vitamin A
Cells
Cultured
Nutrition and Dietetics
Homing Receptor
Forkhead Transcription Factors
hemic and immune systems
Specific Pathogen-Free Organisms
Cell biology
medicine.anatomical_structure
Lymphatic system
Chemoattractant
Female
purl.org/becyt/ford/3 [https]
Lymph
CIENCIAS MÉDICAS Y DE LA SALUD
Receptors
Lymphocyte Homing
Mice
Transgenic
chemical and pharmacologic phenomena
Biology
03 medical and health sciences
purl.org/becyt/ford/3.3 [https]
Immune system
Antigens
CD
Immune Tolerance
medicine
Animals
Molecular Biology
CD86
Lamina propria
Dendritic Cells
Coculture Techniques
Mice
Inbred C57BL
030104 developmental biology
Gene Expression Regulation
Afferent Lymphatic-Dendritic Cell
Dietary Supplements
Immunology
Lymph Nodes
Tolerance
030215 immunology
Homing (hematopoietic)
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Lamina propria dendritic cells (DCs) have a permanent turnover with constitutive migration to mesenteric lymph nodes and replenishment by progenitors. Luminal bacteria and dietary constituents provide key signals that endow DCs their unique properties in vivo. Taking into account that the intestinal immune system is greatly influenced by retinoids, we evaluated in B6 mice 3, 8, 16 and 24 h after feeding a single dose of vitamin A phenotype and function of cells present in mesenteric afferent lymph nodes as well as signals involved in migration. We studied the frequency of CD11c+MHC-II+CD103+CD86+ and RALDH+ DCs by flow cytometry, we determined CCL-21 and D6 levels in tissue homogenates by Western blot, and we co-cultured cells isolated from afferent lymphatics with sorted CD4+ lymphocytes to assess Foxp-3 induction and homing receptor expression. Sixteen hours after vitamin A administration, DCs isolated from afferent lymphatics were able to induce homing receptors and Foxp3 expression in CD4+ lymphocytes. Our results show that a single dose of vitamin A generated a stream of signals and amplified the tolerogenic activity of DCs migrating to lymphoid tissue. Fil: Novotny Núñez, Ivanna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Barrios, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Maccio Maretto, Lisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Correa, Silvia Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Databáze: OpenAIRE
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