A flavonoid-rich extract from bergamot juice prevents carcinogenesis in a genetic model of colorectal cancer, the Pirc rat (F344/NTac-Apcam1137)
Autor: | Giovanna Caderni, Santa Cirmi, Michele Navarra, Andrea Romagnoli, Cristina Luceri, Nadia Ferlazzo, Angelo Pietro Femia, Katia Tortora |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
030109 nutrition & dietetics Nutrition and Dietetics Colorectal cancer Medicine (miscellaneous) Cancer 030209 endocrinology & metabolism Biology medicine.disease_cause medicine.disease Arginase 03 medical and health sciences 0302 clinical medicine In vivo Apoptosis Survivin Genetic model medicine Cancer research Bergamot juice Cancer Citrus bergamia Colon carcinogenesis Inflammation Carcinogenesis |
Zdroj: | European Journal of Nutrition. 59:885-894 |
ISSN: | 1436-6215 1436-6207 |
DOI: | 10.1007/s00394-019-01948-z |
Popis: | To determine the potential of a flavonoid-rich extract from bergamot juice (BJe) to prevent colorectal carcinogenesis (CRC) in vivo. Pirc rats (F344/NTac-Apcam1137), mutated in Apc, the key gene in CRC, were treated with two different doses of BJe (35 mg/kg or 70 mg/kg body weight, respectively) mixed in the diet for 12 weeks. Then, the entire intestine was surgically removed and dissected for histological, immunohistochemical and molecular analyses. Rats treated with BJe showed a significant dose-related reduction in the colon preneoplastic lesions mucin-depleted foci (MDF). Colon and small intestinal tumours were also significantly reduced in rats supplemented with 70 mg/kg of BJe. To elucidate the involved mechanisms, markers of inflammation and apoptosis were determined. Compared to controls, colon tumours from BJe 70 mg/kg-supplemented rats showed a significant down-regulation of inflammation-related genes (COX-2, iNOS, IL-1β, IL-6 and IL-10 and Arginase 1). Moreover, in colon tumours from rats fed with 70 mg/kg BJe, apoptosis was significantly higher than in controls. Up-regulation of p53 and down-regulation of survivin and p21 genes was also observed. These data indicate a strong chemopreventive activity of BJe that, at least in part, is due to its pro-apoptotic and anti-inflammatory actions. This effect could be exploited as a strategy to prevent CRC in high-risk patients. |
Databáze: | OpenAIRE |
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