Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity
| Autor: | K.I. Priyadarsini, Mazhuvancherry Kesavan Unnikrishnan, Pawan G. Nayak, Steve Thomas Pannakal, Piya Paul, Punit Bansal |
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| Rok vydání: | 2011 |
| Předmět: |
DNA damage
Health Toxicology and Mutagenesis Radiation-Protective Agents Pharmacology Toxicology medicine.disease_cause Cell Line Lipid peroxidation Rutin chemistry.chemical_compound Cricetulus Cricetinae medicine TBARS Organic chemistry Animals Humans Cytotoxicity Urticaceae Lung Chromatography High Pressure Liquid Micronucleus Tests Plant Extracts food and beverages Polyphenols General Medicine Free Radical Scavengers Fibroblasts chemistry Polyphenol Gamma Rays Comet Assay Lipid Peroxidation Quercetin Genotoxicity DNA Damage |
| Zdroj: | Environmental toxicology and pharmacology. 33(1) |
| ISSN: | 1872-7077 |
| Popis: | Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against γ-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against γ-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 μg/ml) for radioprotection, PM1 at 25 μg/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 μg/ml), a combination of polyphenols was found most effective in preventing γ-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component. |
| Databáze: | OpenAIRE |
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