Are changes in excitability in the hippocampus of adult male rats induced by prenatal methamphetamine exposure or stress?
| Autor: | Romana Šlamberová, Klára Bernášková, Simona Tomkova |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.medical_treatment Hippocampal formation Hippocampus Methamphetamine Tissue Culture Techniques Random Allocation 03 medical and health sciences Epilepsy 0302 clinical medicine Pregnancy Internal medicine medicine Animals Hippocampus (mythology) Rats Wistar Saline Prenatal methamphetamine exposure Dose-Response Relationship Drug business.industry medicine.disease Disease Models Animal 030104 developmental biology Endocrinology Neurology Prenatal stress Prenatal Exposure Delayed Effects Anesthesia Female Neurology (clinical) business Magnesium Deficiency Stress Psychological 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Epilepsy Research. 137:132-138 |
| ISSN: | 0920-1211 |
| DOI: | 10.1016/j.eplepsyres.2017.08.009 |
| Popis: | Prenatal stress and drug exposure induce permanent alterations of the brain. Even though different brain structures are involved, alterations almost always refer to the hippocampus. The aim of this study was to investigate the excitability of hippocampal slices in low-magnesium epilepsy model of prenatally methamphetamine (MA, 5mg/kg sc.) or saline (sc., stress model) exposed animals in adult male rats. The second aim was to investigate, if a low dose of MA (1ml/kgs.c.) administered in adulthood changes the hippocampal activity of these animals. Adult Wistar male rats were divided into groups according to their prenatal treatment (C - naïve control; Sa - saline; MA - MA administration). One half of the animals was treated with a challenge dose of MA (1mg/kg sc.) 45min before hippocampal slices were cut. The activity of 350μ thick transversal slices of CA1 hippocampi was recorded (latencies of the first epileptiform discharge and the regular epileptiform activity) and evaluated in ACSF with low-magnesium concentration. Effects of prenatal exposure: The highest excitability was found in the Sa (prenatally stressed) group in respect to C and MA groups. This group developed also the highest number of seizure-like events. In addition, the prenatally MA treated group had also higher excitability than C group. Effects of the MA challenge dose: The challenge dose decreased the excitability of prenatally SA- exposed group. To conclude, even a mild prenatal stress significantly increases hippocampal excitability in adulthood and a challenge dose of MA is able to dampen it. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect