Additional file 1 of Interleukin-12 elicits a non-canonical response in B16 melanoma cells to enhance survival

Autor: Byrne-Hoffman, Christina N., Wentao Deng, McGrath, Owen, Wang, Peng, Rojanasakul, Yon, Klinke, David J.
Rok vydání: 2020
DOI: 10.6084/m9.figshare.12367841.v2
Popis: Additional file 1 Supplemental Figure S1: cell subsets were established that vary in expression of iL12RB2 and with minimal fluorescent spillover into pAkt and pSTAT4 measures. (a) Flow cytometric events were subdivided into groups based on IL12RB2 expression, where solid vertical lines denote limits of IL12RB2 expression associated with each subgroup. Red curve denotes distribution in IL12RB2 expression by B16F0 while gray shaded indicates unstained controls. Single-stained controls for IL12RB2 in B16F0 (b) and 2D6 (c) cells were used to establish that fluorescence associated with measuring Akt and STAT4 phosphorylation was not due to fluorescent spillover. Results for two independent experiments are shown for each subgroup based on IL12RB2 expression.
Databáze: OpenAIRE