Biologically Derived, Three-Dimensional, Embryonic Scaffolds for Long-Term Cardiomyocyte Culture
| Autor: | Daniel J. Garry, Mary G. Garry, Stefan M. Kren, Joseph B. Wenger |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Primary (chemistry) Tissue Scaffolds Cell Differentiation macromolecular substances Cell Biology Hematology Biology Embryonic stem cell In vitro Cell biology Mice 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Original Research Reports Animals Myocytes Cardiac 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells Dev |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | The ability to maintain viable cultures of mature, primary cardiomyocytes is challenging. The lack of viable cardiomyocyte cultures severely limits in vitro biochemical assays, toxicology assays, drug screening assays, and other analyses. Here, we describe a novel three-dimensional (3D) embryonic scaffold, which supports the culture of postnatal day 7 murine cardiomyocytes within the embryonic heart for, at least, 28 days. We have observed that these cardiomyocytes display normal differentiation, protein expression, and function after extended culture. This novel culture system will allow for prolonged treatment of cardiomyocytes in a natural 3D orientation and has the potential for providing a superior tool for the screening of therapeutic compounds. |
| Databáze: | OpenAIRE |
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