Fluorocitrate induced the alterations of memory-related proteins and tau hyperphosphorylation in SD rats
| Autor: | Xiu-Qing Yao, Gong-Ping Liu, Quan-Bao Wang, Jia-Yu Zhang, Xiao-Ling Shang, Xiu-Ping Liu, Fu-Yuan Cao, Qun Wang, Jian-Zhi Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Synapsin I Synaptophysin tau Proteins AMPA receptor Rats Sprague-Dawley Glycogen Synthase Kinase 3 Memory medicine Animals Citrates Protein Phosphatase 2 Receptors AMPA Phosphorylation CAMP response element binding Neurons Arc (protein) Glycogen Synthase Kinase 3 beta biology General Neuroscience Intracellular Signaling Peptides and Proteins Brain Membrane Proteins Synapsins Molecular biology Cell biology Enzyme Activation medicine.anatomical_structure nervous system biology.protein Postsynaptic density Disks Large Homolog 4 Protein Astrocyte |
| Zdroj: | Neuroscience letters. 584 |
| ISSN: | 1872-7972 |
| Popis: | Astrocytes provide structural, metabolic and trophic supports for neurons. However, there are no direct evidences whether astrocytes involve in the regulation of synaptic proteins expression and tau phosphorylation until now. Here, we injected 1 nmol fluorocitrate (FC), which preferentially taken up by astrocytes and results in reversible inhibition of the astrocytic tricarboxylic acid cycle, into the left lateral ventricle of the brain in the SD rats for 1h, and found that FC treatment decreased several memory-related proteins levels, such as AMPA receptor GluR1/2, postsynaptic density protein 93/95, Arc and phosphorylated cAMP response element binding proteins, while increased synaptophysin and synapsin I levels in the hippocampus. FC treatment also increased the levels of phosphorylated tau at multiple Alzheimer-related phosphorylation sites, as well as activation of glycogen synthase kinase-3β and inactivation of protein phosphatase-2A. Similar effects were also observed in the primary hippocampal neurons, which were cultured with the conditioned media from FC-treatment primary astrocytes. Our data suggest that astrocytes regulate neuronal tau phosphorylation and several synaptic proteins expression. |
| Databáze: | OpenAIRE |
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