Purification and inflammatory edema induced by two PLA2 (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae)
| Autor: | Daniel Martins-de-Souza, José Antonio Valeriano-Zapana, Frank Denis Torres-Huaco, Sergio Marangoni, Queila Cristina Dias, Elen Cristina Teizem Landucci, Fábio André Marangoni, Augusto Vilca-Quispe, Luis Alberto Ponce-Soto |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Physiology Molecular Sequence Data Mollusk Venoms Sequence alignment Biology Sea anemone Biochemistry Homology (biology) Sequence Analysis Protein Botany Animals Edema Amino Acid Sequence Rats Wistar Molecular Biology Peptide sequence Skin Chromatography Reverse-Phase Molecular mass Foot Anthothoe chilensis equipment and supplies biology.organism_classification Rats Phospholipases A2 Sea Anemones Sephadex Snake venom Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteolysis lipids (amino acids peptides and proteins) Sequence Alignment |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 161:170-177 |
| ISSN: | 1096-4959 |
| Popis: | The Anch TX-I and II PLA(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on μ-Bondapak C18 column. Both PLA(2) showed a molecular mass of ~14kDa determined by MALDI-TOF mass spectrometry and showed a high catalytic activity (data not showed). Although homologous with mammalian or snake venom group I PLA(2)s, Anch TX-I and II is sufficiently structurally different for the question of its placement into the existing PLA(2) classification scheme to arise. In addition, Anch TX-I and II, despite possessing many common structural features, also differ in some important structural properties. The amino acid sequence of both PLA(2) (Anch TX-I and III) showed high amino acid sequence identity with PLA(2)Rhopilema nomadica and Bunodosoma caissarum Cnidaria and PLA(2) of group III protein isolated from the Mexican lizard Heloderma horridum horridum and Heloderma suspectum. In addition, Anch TX-I and Anch TX-II showed high amino acid sequence identity with PLA(2) from group III also showed significant overall homology to bee Apis dorsata, Bombus terrestris and Bombus pennsylvanicus and PLA(2). We also investigated the in vivo edematogenic activity of Anch TX-I and Anch TX-II in a model of paw and skin edema in rats and observed that both are able to induce dose-dependent edema. |
| Databáze: | OpenAIRE |
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