Effects of venlafaxine on p90Rsk activity in rat C6-gliomas and brain
| Autor: | Jin-Jun Liang, Stephen M. Storm, Xavier Z. Khawaja |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
MAPK/ERK pathway
Agonist Male medicine.medical_specialty medicine.drug_class Central nervous system Venlafaxine Biology Hippocampal formation Ribosomal Protein S6 Kinases 90-kDa Rats Sprague-Dawley In vivo Internal medicine Cell Line Tumor medicine Animals Dose-Response Relationship Drug General Neuroscience Venlafaxine Hydrochloride Brain Glioma Cyclohexanols Rats Endocrinology medicine.anatomical_structure Antidepressant Intracellular medicine.drug |
| Zdroj: | Neuroscience letters. 372(1-2) |
| ISSN: | 0304-3940 |
| Popis: | The intracellular actions of the antidepressant, venlafaxine, were studied in C6-gliomas using a phosphoproteomics approach. Long-term pre-treatment of C6-gliomas with venlafaxine followed by an acute challenge with isoproterenol (a β-adrenoceptor agonist), resulted in increased p90Rsk phosphorylation (three-fold) versus control levels (isoproterenol alone). The effect of venlafaxine pre-treatment on p90Rsk activity was dose-dependent (EC 50 = 3.75 nM) in C6 gliomas. In rat brain sections, intense immunoreactive phospho-p90Rsk labeling was observed for both neurons and glia, especially in cortical layers II/III and hippocampal formations. In vivo studies demonstrated an intense but similar distribution pattern of phospho-p90Rsk staining after chronic venlafaxine dosing of rats compared to naives and no region-specific drug effect was observed in vivo. In conclusion, our findings suggest that some of the centrally-mediated benefits of venlafaxine in depression may be due to its intracellular properties especially on the neuro-glial circuitry and MAPK/p90Rsk-dependent pathways at an early stage. |
| Databáze: | OpenAIRE |
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