A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics
| Autor: | Norbert Polacek, Ioan Iacovache, Julia Reuther, Walid H. Gharib, Andreas Pircher, Benoît Zuber, Lukas Schneider |
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| Rok vydání: | 2021 |
| Předmět: |
translation control
RNA Untranslated Saccharomyces cerevisiae 610 Medicine & health Biology Ribosome 03 medical and health sciences 0302 clinical medicine RNA Transfer Large ribosomal subunit 540 Chemistry Protein biosynthesis Nucleotide RNA Messenger Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences rancRNA Translation (biology) l1 stalk Cell Biology Non-coding RNA biology.organism_classification Cell biology ribosome chemistry Protein Biosynthesis 030220 oncology & carcinogenesis Transfer RNA 570 Life sciences biology Non-coding rna Ribosomes Research Article Research Paper |
| Zdroj: | RNA Biology article-version (VoR) Version of Record Reuther, Julia; Schneider, Lukas; Iacovache, Ioan; Pircher, Andreas; Gharib, Walid H.; Zuber, Benoît; Polacek, Norbert (2021). A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics. RNA biology, 18(12), pp. 2617-2632. Taylor and Francis 10.1080/15476286.2021.1935573 |
| ISSN: | 1555-8584 1547-6286 |
| DOI: | 10.1080/15476286.2021.1935573 |
| Popis: | Ribosome-associated non-coding RNAs (rancRNAs) have been recognized as an emerging class of regulatory molecules capable of fine-tuning translation in all domains of life. RancRNAs are ideally suited for allowing a swift response to changing environments and are therefore considered pivotal during the first wave of stress adaptation. Previously, we identified an mRNA-derived 18 nucleotides long rancRNA (rancRNA_18) in Saccharomyces cerevisiae that rapidly downregulates protein synthesis during hyperosmotic stress. However, the molecular mechanism of action remained enigmatic. Here, we combine biochemical, genetic, transcriptome-wide and structural evidence, thus revealing rancRNA_18 as global translation inhibitor by targeting the E-site region of the large ribosomal subunit. Ribosomes carrying rancRNA_18 possess decreased affinity for A-site tRNA and impaired structural dynamics. Cumulatively, these discoveries reveal the mode of action of a rancRNA involved in modulating protein biosynthesis at a thus far unequalled precision. |
| Databáze: | OpenAIRE |
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