Effect of Heparinoid on Free Thyroxine Concentration in Human Serum
| Autor: | Sumio Tayama, Toshiro Sakurada, Shintaro Saito, Tatsuo Torikai |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Blood Glucose
Electrophoresis Male medicine.medical_specialty Protamine sulfate Heparinoid Palmitic Acids Fatty Acids Nonesterified Hyperthyroidism General Biochemistry Genetics and Molecular Biology Iodine Radioisotopes Palmitic acid Thyroxine-Binding Proteins chemistry.chemical_compound Thyroxine-binding globulin Dogs Hypothyroidism Internal medicine medicine Animals Humans Euthyroid Carbon Radioisotopes Protamines Binding site biology Albumin Dextrans General Medicine In vitro Thyroxine Endocrinology Heparinoids chemistry Injections Intravenous biology.protein Iodine medicine.drug |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 110:209-224 |
| ISSN: | 1349-3329 0040-8727 |
| Popis: | Sodium salt of dextran sulfate ester (MDS-Kowa, Kowa Co., Japan) was administered intravenously to 4 euthyroid subjects, 7 patients with hyperthyroidism and 5 patients with hypothyroidism. Serum FFA rose to the level 4-6 times higher than the preinjection level 15min after the intravenous injection. At the same time, elutable fraction (EF) of T4, free T4, and binding capacity and concentration of TBPA increased, while binding capaty of TBG decreased. Addition of dextran sulfate to serum in vitro caused no change in binding capacity of TBG and EF of T4. Addition of palmitic acid to serum in vitro with increasing concentration resulted in a gradual decrease in the distribution of 131I-T4 to TBG and binding capacity of TBG, while EF of T4 gradually increased. Intravenous injection of dextran sulfate ester following the prior injection of protamine sulfate in dog caused a mild rise in serum FFA, but failed to increase the EF of T4. When reverse-flow paper electrophoresis was carried out after addition of 14C-palmitic acid to sera of normal and TBG-deficient subjects, it was confirmed that palmitic acid bound to albumin and TBG fraction. These results suggest that dextran sulfate ester raises serum FFA first, then the increased FFA combines with TBP at least with TBG, thus competing for binding sites with T4, and released T4 in turn increases free T4 level in the serum. |
| Databáze: | OpenAIRE |
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