Induction of somatic hypermutation by antigen-specific B cell receptors in the human BL2 cell line

Autor: Florence Glaudet, Vincent Denis, Michel Cogné, Ahmed Amine Khamlichi
Přispěvatelé: Physiologie Moléculaire de la Réponse Immune et des Lymphoproliférations (PMRIL), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2004
Předmět:
MESH: Signal Transduction
Gene Rearrangement
B-Lymphocyte
Heavy Chain
MESH: Amino Acid Sequence
MESH: Base Sequence
MESH: Down-Regulation
Mice
MESH: Reverse Transcriptase Polymerase Chain Reaction
Immunology and Allergy
MESH: Animals
MESH: Gene Rearrangement
B-Lymphocyte
Heavy Chain
Genes
Immunoglobulin
Reverse Transcriptase Polymerase Chain Reaction
breakpoint cluster region
Immunoglobulin genes
Stop codon
Immunoglobulin Isotypes
MESH: Immunoglobulin Class Switching
MESH: Immunologic Memory
[SDV.IMM]Life Sciences [q-bio]/Immunology
Signal Transduction
MESH: Somatic Hypermutation
Immunoglobulin
NLM
MESH: Cell Line
Tumor
Immunology
B-cell receptor
Molecular Sequence Data
Somatic hypermutation
Down-Regulation
Receptors
Antigen
B-Cell
Biology
Transfection
MESH: Receptors
Antigen
B-Cell
Antigen
MESH: RNA
Cell Line
Tumor
Animals
Humans
Amino Acid Sequence
Gene
MESH: Mice
MESH: Molecular Sequence Data
MESH: Humans
B cell receptor
Base Sequence
MESH: Transfection
Molecular biology
Immunoglobulin Class Switching
B-1 cell
MESH: Genes
Immunoglobulin
Cell culture
MESH: Immunoglobulin Isotypes
MESH: Muramidase
RNA
Muramidase
Class-switched isotypes
Somatic Hypermutation
Immunoglobulin
Immunologic Memory
Zdroj: European Journal of Immunology
European Journal of Immunology, Wiley-VCH Verlag, 2004, 34 (6), pp.1637-45. ⟨10.1002/eji.200324741⟩
ISSN: 0014-2980
1521-4141
DOI: 10.1002/eji.200324741⟩
Popis: The role of the B cell antigen receptor in the induction of somatic hypermutation is presently unclear. We established stable transfectants of the human BL2 cell line expressing hen-egg lysozyme-specific IgM or IgA and compared their ability to induce somatic hypermutation of the endogenous rearranged heavy-chain gene. We found that IgM and IgA were both able to induce somatic hypermutation in an antigen dose-independent manner. The mutations displayed most of the characteristics of somatic hypermutation in vivo. Notably, some replacements introduced stop codons in the coding region. Our data suggest that class-switched memory B cells may undergo somatic hypermutation. They also suggest that the transmembrane/cytoplasmic domains of the class-switched isotypes modulate the signaling and down-modulation activities of the BCR in an antigen dose-dependent manner.
Databáze: OpenAIRE
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