Fluorogenic hand-held immunoassay for the identification of ricin: rapid analyte measurement platform

Autor:	R. E. Fulton, H. G. Thompson
Rok vydání:	2007
Předmět:	Detection limit Immunoassay Reproducibility Analyte Chromatography Time Factors medicine.diagnostic_test Clinical Biochemistry Immunology Hand held Reproducibility of Results Ricin Sensitivity and Specificity Fluorescence Medical Laboratory Technology chemistry.chemical_compound Sample volume chemistry medicine Immunology and Allergy Biological agent
Zdroj:	Journal of immunoassayimmunochemistry. 28(3)
ISSN:	1532-1819
Popis:	Rapid analyte measurement platform (RAMPtrade mark) fluorogenic hand-held immunoassays (HHAs) were evaluated for inclusivity/sensitivity, exclusivity/specificity, sample matrix effects, ruggedness/stability, and reproducibility in detection of ricin (RCA(60)), a potential biological threat agent. The limit of detection of HHAs for RCA(60) was 14 ng/mL or approximately 140 pg/test. HHAs were inclusive in detection of ricin RCA(60), RCA(120), ricin A chain, and ricin B chain and exclusive in discrimination of RCA(60) from other toxins. None of the sample matrices tested affected assay performance. RAMPtrade mark ratios were tolerant of increases in sample volume, however, a decrease in sample volume of 25% resulted in significantly increased readings. RAMPtrade mark readings were highly reproducible lot-to-lot, cartridge-to-cartridge, day-to-day, and reader-to-reader.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::407a2332130fe2b00f4d6a08f34b78c3 https://pubmed.ncbi.nlm.nih.gov/17613669 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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