Biosynthesis of the Escherichia coli K5 polysaccharide, a representative of group II capsular polysaccharides: polymerization in vitro and characterization of the product
| Autor: | A V Nikolaev, Barbara Jann, Dorothea Bronner, Klaus Jann, Andreas Finke |
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| Rok vydání: | 1991 |
| Předmět: |
Bacterial capsule
Macromolecular Substances Polysaccharide medicine.disease_cause Microbiology Sugar acids chemistry.chemical_compound Biosynthesis Escherichia coli medicine Molecular Biology Bacterial Capsules chemistry.chemical_classification Antigens Bacterial biology Tunicamycin Cell Membrane Polysaccharides Bacterial Sugar Acids biology.organism_classification Molecular biology Enterobacteriaceae Kinetics Membrane chemistry Biochemistry Glucosyltransferases Bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 173:4088-4094 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.173.13.4088-4094.1991 |
| Popis: | Biosynthesis of the capsular K5 polysaccharide of Escherichia coli, which has the structure 4)-beta GlcA-1,4-alpha GlcNAc-(1, was studied with membrane preparations from an E. coli K5 wild-type strain and from a recombinant K-12 strain expressing the K5 capsule. Polymerization occurs at the inner face of the cytoplasmic membrane without the participation of lipid-linked oligosaccharides. The serological K5 specificity of the in vitro product was determined with a K5-specific monoclonal antibody in an antigen-binding assay. The K5 polysaccharide, as obtained from the membranes after an in vitro incubation, has 2-keto-3-deoxyoctulosonic acid as the reducing sugar, which indicates that the polysaccharide grows by chain elongation at the nonreducing end. |
| Databáze: | OpenAIRE |
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