Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in IGHD Haploinsufficient Humans
| Autor: | Louis Boon, Menno C. van Zelm, Marcela Vlková, Zita Chovancová, Sophinus J. W. Bartol, Jana Nechvatalova |
|---|---|
| Přispěvatelé: | Immunology |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Somatic cell Cellular differentiation Immunology Naive B cell hemic and immune systems chemical and pharmacologic phenomena Biology Immunoglobulin D Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine stomatognathic system Immunoglobulin class switching Antigen immune system diseases hemic and lymphatic diseases biology.protein Immunology and Allergy IGHD Memory B cell 030215 immunology |
| Zdroj: | Journal of Immunology, 201(7), 1928-1935. American Association of Immunologists |
| ISSN: | 0022-1767 |
| Popis: | Surface IgD is coexpressed with IgM on naive mature B cells. Still, the role of surface IgD remains enigmatic even 50 y after its initial discovery. In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD− naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD− subset. Furthermore, both IgD+ and IgD− naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class–switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|