Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster

Autor:	Atsushi, Kojima, Nanako, Osawa, Mami, Oba, Yukie, Katayama, Tsutomu, Omatsu, Tetsuya, Mizutani
Rok vydání:	2022
Předmět:	General Veterinary DNA Ribosomal Spacer Saccharomycetales Animals DNA Intergenic DNA Ribosomal Phylogeny
Zdroj:	Journal of Veterinary Medical Science. 84:244-250
ISSN:	1347-7439 0916-7250
Popis:	Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region from avian feces. Phylogenetic analysis of MO collected from Japanese pet birds showed little genetic variation; analysis based on these regions did not distinguish between host species order, differences in MO shape, or host gastrointestinal symptoms. These regions were found to be unsuitable for molecular epidemiological studies of MO and further investigation into other genetic regions is required.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40746485f291cf33e1bdc6ce2691887c https://doi.org/10.1292/jvms.21-0576 Zobrazit plný text záznamu