Dissecting high from low responders in a vitamin D3 intervention study
| Autor: | Antonio Neme, Tomi-Pekka Tuomainen, Matti Uusitupa, Jyrki K. Virtanen, Tarja Nurmi, Sari Voutilainen, Carsten Carlberg, Vanessa D. de Mello, Noora Saksa, Jussi Ryynänen |
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| Rok vydání: | 2014 |
| Předmět: |
Vitamin
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Parathyroid hormone Biology Biochemistry Calcitriol receptor Biomarkers Pharmacological Transcriptome Prediabetic State 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Internal medicine medicine Vitamin D and neurology Humans NRIP1 Molecular Biology 030304 developmental biology Cholecalciferol 0303 health sciences Insulin Cell Biology Vitamins 3. Good health chemistry Gene Expression Regulation 030220 oncology & carcinogenesis Multigene Family Homeostatic model assessment Leukocytes Mononuclear Molecular Medicine Receptors Calcitriol Signal Transduction Transcription Factors |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 148 |
| ISSN: | 1879-1220 |
| Popis: | Vitamin D3 is a pleiotropic signaling molecule that has via activation of the transcription factor vitamin D receptor (VDR) a direct effect on the expression of more than 100 genes. The aim of this study was to find transcriptomic and clinical biomarkers that are most suited to identify vitamin D3 responders within 71 pre-diabetic subjects during a 5-month intervention study (VitDmet). In hematopoietic cells, the genes ASAP2, CAMP, CD14, CD97, DUSP10, G0S2, IL8, LRRC8A, NINJ1, NRIP1, SLC37A2 and THBD are known as primary vitamin D targets. We demonstrate that each of these 12 genes carries a conserved VDR binding site within its genomic region and is expressed in human peripheral blood mononuclear cells (PBMCs). The changes in the expression of these genes in human PBMCs at the start and the end of the vitamin D-intervention were systematically correlated with the alteration in the circulating form of vitamin D3, 25-hydroxyvitamin D3 (25(OH)D3). Only 39-44 (55-62%) of the study subjects showed a highly significant response to vitamin D3, i.e., we considered them as "responders". In comparison, we found for 37-53 (52-75%) of the participants that only 12 biochemical and clinical parameters, such as concentrations of parathyroid hormone (PTH) and insulin, or computed values, such as homeostatic model assessment and insulin sensitivity index, show a correlation with serum 25(OH)D3 levels that is as high as that of the selected VDR target genes. All 24 parameters together described the pleiotropic vitamin D response of the VitDmet study subjects. Interestingly, they demonstrated a number of additional correlations that define a network, in which PTH plays the central role. In conclusion, vitamin D3-induced changes in human PBMCs can be described by transcriptomic and serum biomarkers and allow a segregation into high and low responders. This article is part of a Special Issue entitled '17th Vitamin D Workshop' . |
| Databáze: | OpenAIRE |
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